Parallel studies confirm Francisella halioticida causes mortality in Yesso scallops Patinopecten yessoensis
Parallel studies confirm Francisella halioticida causes mortality in Yesso scallops Patinopecten yessoensis
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DOI：
10.3354/dao03383
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发表时间：
2019-01-01
影响因子：
1.4
通讯作者：
Itoh, Naoki
中科院分区：
文献类型：
--
作者：
Kawahara, Miku;Meyer, Gary R.;Itoh, Naoki
杀盐弗朗西斯菌是一种海洋细菌，最初被描述为导致大规模死亡的病原体。最近在加拿大和日本的实地研究表明，这种细菌也是导致虾夷扇贝闭壳肌病变和高死亡率的原因，尽管因果关系尚未确定。本研究对F.在加拿大和日本，使用从每个国家的患病Yesso扇贝中分离的细菌评估了Yesso扇贝中的嗜盐菌。独立的实验室实验表明，扇贝感染F。通过浴暴露杀盐线虫导致高死亡率和以大量血细胞浸润为特征的组织学损伤。F.用PCR方法鉴定出嗜盐菌（Halioticida），F.从部分死亡和存活的标本中重新分离嗜盐菌。这些结果满足科赫的经典标准，建立疾病的因果关系，并提供了确凿的证据，F。嗜盐菌引起虾夷扇贝闭壳肌损伤和高死亡率。
Francisella halioticida is a marine bacterium originally described as the causative agent of mass mortality among giant abalone Haliotis gigantea. Recent field studies in Canada and Japan have suggested that this bacterium is also the cause of adductor muscle lesions and high mortality of Yesso scallops Patinopecten yessoensis, although a causal relationship has not been established. In the present study, the pathogenicity of F. halioticida in Yesso scallops was assessed in both Canada and Japan using bacteria isolated from diseased Yesso scallops in each respective country. Independent laboratory experiments revealed that scallops challenged with F. halioticida via bath exposure resulted in high mortality and histological lesions characterized by massive haemocyte infiltration. The presence of F. halioticida was confirmed using PCR, and F. halioticida was re-isolated from a portion of dead and surviving specimens. These results fulfill Koch's classic criteria for establishing disease causation and provide conclusive evidence that F. halioticida causes adductor muscle lesions and high mortality in Yesso scallops.