Localization of endocrine pancreatic islets
Localization of endocrine pancreatic islets
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DOI：
10.1007/s002689900155
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发表时间：
1996-10-01
影响因子：
2.6
通讯作者：
Hardy, MA
中科院分区：
文献类型：
--
作者：
Fiedor, PS;Oluwole, SF;Hardy, MA
全胰腺或胰岛移植仍然是治疗糖尿病的一种有前途的方法。由于目前尚无有效的体内早期诊断胰岛排斥反应或胰腺内分泌功能紊乱的方法，我们探讨了双硫腙(DTZ)及其合成的碘衍生物(I-DTZ)能否作为监测移植胰岛存活的潜在放射性标志物。取自多器官供者的人胰岛，在胰管内注射DTZ溶液进行胰岛染色后，进行体外检测。在体内实验中，Lewis大鼠静脉注射不同浓度的DTZ或I-DTZ后，对胰岛进行原位定位。新鲜的大鼠胰岛被移植到链脲佐菌素诱导的糖尿病受体中，要么移植到肾被膜下，要么移植到腹膜内。然后用静脉注射DTZ或I-DTZ对存活的移植胰岛进行宏观和微观鉴定。这些研究表明，DTZ和I-DTZ溶液在静脉注射后对体内的胰岛进行特异性染色，而不会损害其内分泌功能，通过血浆胰岛素和血糖水平进行评估。人的胰岛在体外研究中染成红色，类似于大鼠的DTZ(I-DTZ)效应。结论DTZ和I-DTZ在体内和体外鉴定胰岛是有效的，可能在胰岛肿瘤(胰岛素瘤)的检测和胰腺器官移植或胰岛排斥反应的诊断中有潜在的临床应用价值。
Transplantation of whole pancreas or pancreatic islets remains a promising approach to treatment of diabetes mellitus. Because at present there is no efficient method for in vivo early diagnosis of pancreatic islet rejection or for disorders of pancreatic endocrine function, we examined if dithizone (DTZ) and a synthetic iodo-derivative of DTZ (I-DTZ) can he used as a potential radioactive marker for monitoring viable transplanted pancreatic islets. Human pancreatic islets harvested from multiorgan donors were tested ex vivo after intraductal injection of DTZ solution for islet staining. Lewis rats Irere used in the in vivo experiments for localizing pancreatic islets in situ after intravenous injection of various concentrations of DTZ or I-DTZ. Fresh rat islets were transplanted into streptozotocin-induced diabetic recipients, either underneath the kidney capsule or intraperitoneally. Intravenous DTZ or I-DTZ was then used for macroscopic and microscopic identification of viable transplanted islets. These studies indicate that DTZ and I-DTZ solutions specifically stain pancreatic islets in vivo after intravenous injection without damage to their endocrine function as assessed by plasma insulin and glucose levels. Human islets stain red in in vitro studies similar to the DTZ (I-DTZ) effect in rats. We conclude that DTZ and I-DTZ are effective in the in vivo and in vitro identification of pancreatic islets and may have potential clinical application in the detection of pancreatic islet tumors (insulinomas) and in the diagnosis of rejection of pancreatic organ allografts or islets.