Modelling protein crystallisation using morphological population balance models
Modelling protein crystallisation using morphological population balance models
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DOI：
10.1016/j.cherd.2009.08.015
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发表时间：
2010-04-01
影响因子：
3.9
通讯作者：
Wang, Xue Z.
中科院分区：
文献类型：
--
作者：
Liu, Jing J.;Ma, Cai Y.;Wang, Xue Z.
已知蛋白质结晶受到许多因素的影响并且固有地难以控制。能够模拟晶体生长行为，特别是在结晶器中颗粒群的工艺规模下，无疑将极大地帮助蛋白质晶体的配制和受控制造。本文提出了一种适用于四氢鸡卵白色（HEW）溶菌酶结晶过程的形态学群体平衡模型。由于粒子数平衡模型包含了晶体的形状信息，因此它能够模拟晶体形状分布和尺寸分布的动态演化。形态学群体平衡模型需要刻面生长动力学数据，其从文献中关于HEW溶菌酶晶体的两个鉴定的独立晶面{101}和{110}的公开数据获得。(C)2009年，化学工程师学会。Elsevier B. V.出版，保留所有权利。
Protein crystallisation is known to be affected by many factors and inherently difficult to control. Being able to model the crystal growth behaviour, especially at process scale for the population of particles in a crystalliser will no doubt greatly help the formulation and controlled manufacture of protein crystals. In this paper, a morphological population balance model for crystallisation of tetragonal Hen-Egg-White (HEW) lysozyme is presented. Since the population balance model has incorporated crystal shape information, it is able to simulate the dynamic evolution of the shape distribution as well as size distribution. The morphological population balance model requires faceted growth kinetics data, which was obtained from published data in literature for the two identified independent crystallographic faces, {101} and {110}, of HEW lysozyme crystals. (C) 2009 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.