miR-206 regulates the growth of the teleost tilapia (Oreochromis niloticus) through the modulation of IGF-1 gene expression
miR-206 regulates the growth of the teleost tilapia (Oreochromis niloticus) through the modulation of IGF-1 gene expression
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DOI：
10.1242/jeb.079590
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发表时间：
2013-04-01
影响因子：
2.8
通讯作者：
Zhao, Jin-Liang
中科院分区：
文献类型：
--
作者：
Yan, Biao;Zhu, Chang-Dong;Zhao, Jin-Liang
MicroRNAs （miRNAs）类似于22核苷酸的非编码rna，在调节肌肉发育中起着至关重要的作用。我们前期的研究表明，miR-206在罗非鱼骨骼肌中特异性表达，并在不同发育阶段呈现动态表达模式。在这里，我们发现miR-206是罗非鱼生长的关键调节因子。miR-206功能丧失导致罗非鱼生长加速。IGF-1被确定为miR-206的靶基因。miR-206通过靶向IGF-1的3'UTR直接改变其表达，抑制miR-206可显著提高体内IGF-1 mRNA水平。因此，miR-206可以作为一种分子标记物来辅助鱼类育种。
MicroRNAs (miRNAs) are similar to 22-nucleotide noncoding RNAs that play a crucial role in regulating muscle development. Our previous study shows that miR-206 is specifically expressed in tilapia skeletal muscle, and exhibits a dynamic expression pattern at different developmental stages. Here, we reveal that miR-206 emerges as a crucial regulator of tilapia growth. miR-206 loss of function leads to the acceleration of tilapia growth. IGF-1 is identified as the target gene of miR-206. miR-206 directly changes IGF-1 expression by targeting its 3'UTR, and inhibition of miR-206 substantially increases the IGF-1 mRNA level in vivo. Thus, miR-206 could be developed as a molecular marker to assist fish breeding.