Gene expression analysis in HBV transgenic mouse liver: A model to study early events related to hepatocarcinogenesis
Gene expression analysis in HBV transgenic mouse liver: A model to study early events related to hepatocarcinogenesis
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DOI：
10.2119/2006-00015.barone
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发表时间：
2006-04-01
影响因子：
5.7
通讯作者：
Iolascon, Achille
中科院分区：
文献类型：
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作者：
Barone, Michele;Spano, Daniela;Iolascon, Achille
乙肝病毒是导致肝细胞癌发生的主要病因之一。我们使用一只含有乙肝病毒序列的转基因小鼠作为模型系统来揭示乙肝病毒诱发肝癌的分子机制。我们之所以选择这个动物模型，是因为在模拟人类感染乙肝病毒的自然历史的中间步骤之后，它会持续发展为肝癌。在这项研究中，我们关注导致肝癌的早期事件。我们比较了3月龄转基因小鼠和3月龄野生型(Wt)动物的基因表达谱。在转基因小鼠中，基因芯片数据分析显示，共有45个显著差异表达的基因，25个高表达(折叠变化。2；P=0.0025)，20个下调(折叠式变化
Hepatitis B virus (HBV) is one of the major etiological factors responsible for the development of hepatocellular carcinoma (HCC). We used a transgenic mouse, containing HBV sequences, as a model system to unravel the molecular mechanisms of hepatocarcinogenesis induced by HBV. We chose this animal model because it consistently develops liver cancer after intermediate steps that mimic the natural history of HBV infection in humans. In this study, we focus our attention on the early events leading to liver cancer. We compared the gene expression profile of 3-month-old transgenic mice with that of 3-month-old wild-type (wt) animals. In the transgenic mouse, microarray data analysis showed a total of 45 significantly differentially expressed genes, 25 highly expressed (fold change. 2; P = 0.0025), and 20 downregulated (fold change