A Photoactivatable BODIPY Probe for Localization-Based Super-Resolution Cellular Imaging
A Photoactivatable BODIPY Probe for Localization-Based Super-Resolution Cellular Imaging
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DOI：
10.1002/anie.201805827
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发表时间：
2018-09-24
影响因子：
16.6
通讯作者：
Smith, Emily A.
中科院分区：
文献类型：
--
作者：
Wijesooriya, Chamari S.;Peterson, Julie A.;Smith, Emily A.
报道了一种可光活化的硼烷基化BODIPY探针的合成和应用。探针的光激活和激发是通过一个以前未知的低功率可见激光的硼光脱烷基反应来实现的，而不需要在成像缓冲液中添加还原剂或氧清除剂。这些特点导致了一个多功能探针的定位为基础的显微镜的生物系统。探针可以很容易地连接到含核分子的目标特定细胞器。通过将紫杉醇附着在可光激活的BODIPY上，证明了微管的体外和体内超分辨率成像。这是使用可见光激活的BODIPY化合物作为荧光探针的基于单分子定位的超分辨率显微镜的第一个例子。
The synthesis and application of a photoactivatable boron-alkylated BODIPY probe for localization-based super-resolution microscopy is reported. Photoactivation and excitation of the probe is achieved by a previously unknown boron-photodealkylation reaction with a single low-power visible laser and without requiring the addition of reducing agents or oxygen scavengers in the imaging buffer. These features lead to a versatile probe for localization-based microscopy of biological systems. The probe can be easily linked to nucleophile-containing molecules to target specific cellular organelles. By attaching paclitaxel to the photoactivatable BODIPY, invitro and invivo super-resolution imaging of microtubules is demonstrated. This is the first example of single-molecule localization-based super-resolution microscopy using a visible-light-activated BODIPY compound as a fluorescent probe.