Comparison of diagnostic techniques for Helicobacter cetorum infection in wild Atlantic bottlenose dolphins (Tursiops truncatus)
Comparison of diagnostic techniques for Helicobacter cetorum infection in wild Atlantic bottlenose dolphins (Tursiops truncatus)
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DOI：
10.1128/jcm.41.7.2842-2848.2003
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发表时间：
2003-07-01
影响因子：
9.4
通讯作者：
Fox, JG
中科院分区：
文献类型：
--
作者：
Harper, CG;Whary, MT;Fox, JG
从大西洋白海豚胃和太平洋白海豚粪便中分离出一种新的螺旋杆菌。海豚和白鲸（Delphinapterus leucas）。H. 16S rRNA测序显示，cetorum与幽门螺杆菌具有高度同源性，而H.鲸类感染与鲸目动物的胃炎和临床症状有关。由于H. cetorum在野生种群中是未知的，用于检测H.作为一项长期健康研究的一部分，对20只野生长吻海豚进行了比较。通过培养、Southern印迹和PCR检测粪便样品中的Helicobacter，使用属特异性和H。鲸蜡特异性引物。用酶联免疫吸附试验（ELISA）检测H。鲸免疫球蛋白G（IgG）。H. 20份粪便标本中有4份培养出H.cetorum，其中7份为幽门螺杆菌阳性，8份为H. cetorum使用物种特异性引物。另外两份粪便样本经螺杆菌Southern印迹检测呈阳性，表明感染了另一种螺杆菌。所有20份血清均含有高水平的H. cetorum，显着降低血清与全细胞悬浮液的H.脑皮质（P < 0.02）。在血清ELISA的特异性可以通过检测确认未受感染的海豚的血清来确定之前，粪便的PCR和Southern印迹筛选是检测H. cetorum，结果表明，至少有50%的流行H。感染了鲸蜡病毒
Helicobacter cetorum sp. nov. has been cultured from the stomach of Atlantic white-sided dolphins (Lagenorhynchus acutus) and the feces of Pacific white-sided (L. obliquidens) and Atlantic bottlenose (Tursiops truncatus) dolphins and a beluga whale (Delphinapterus leucas). H. cetorum has high homology to Helicobacter pylori as shown by 16S rRNA sequencing, and H. cetorum infection has been associated with gastritis and clinical signs in cetaceans. Because the prevalence of H. cetorum in wild populations is unknown, minimally invasive techniques for detecting H. cetorum were compared for 20 wild bottlenose dolphins sampled as part of a long-term health study. Fecal samples were tested for helicobacter by culture, Southern blotting, and PCR using genus-specific and H. cetorum-specific primers. An enzyme-linked immunosorbent assay (ELISA) was developed to measure H. cetorum immunoglobulin G (IgG). H. cetorum was cultured from 4 of 20 fecal samples, 7 samples were positive using Helicobacter sp. PCR, and 8 samples were positive for H. cetorum using species-specific primers. Two additional fecal samples were positive by Helicobacter sp. Southern blotting, suggesting infection with another helicobacter. All 20 sera contained high levels of IgG antibodies to H. cetorum that were significantly lowered by preabsorption of the sera with whole-cell suspensions of H. cetorum (P < 0.02). Until the specificity of the serum ELISA can be determined by testing sera from dolphins confirmed to be uninfected, PCR and Southern blot screenings of feces are the most sensitive techniques for detection of H. cetorum, and results indicate there is at least a 50% prevalence of H. cetorum infection in these dolphins.