Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies
Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies
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DOI：
10.1016/j.mcp.2005.09.003
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发表时间：
2006-02-01
影响因子：
3.3
通讯作者：
Sachse, K
中科院分区：
文献类型：
--
作者：
Ehricht, R;Slickers, P;Sachse, K
本研究旨在确定优化后的DNA微阵列检测衣原体的检出限和种类鉴定。对携带沙眼衣原体靶序列和该生物基因组DNA的质粒标准品稀释系列的检查显示，单个pcr扩增的靶拷贝足以获得特定的杂交模式。这种性能使该测试适用于临床样品的常规测试。(c) 2005 Elsevier Ltd版权所有。
This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples. (c) 2005 Elsevier Ltd. All rights reserved.