Integrated analysis of long noncoding RNAs and mRNA expression profiles reveals the potential role of lncRNAs in early stage of post-peripheral nerve injury in Sprague-Dawley rats.
Integrated analysis of long noncoding RNAs and mRNA expression profiles reveals the potential role of lncRNAs in early stage of post-peripheral nerve injury in Sprague-Dawley rats.
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长非编码RNA和mRNA表达谱的综合分析揭示了lncRNA在Sprague-Dawley大鼠周围神经损伤后早期的潜在作用。
DOI：
10.18632/aging.202989
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发表时间：
2021-05-08
期刊：
影响因子：
--
通讯作者：
He B
中科院分区：
文献类型：
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作者：
Zhang Y;Zhu Z;Liu X;Xu S;Zhang Y;Xu Y;He B
LncRNAs在周围神经损伤(PNI)后早期的调控作用尚不清楚。本研究利用下一代测序技术对大鼠坐骨神经损伤后第4天(D4)和第7天(D7)的正常坐骨神经(对照组)和损伤组织进行了深度测序。分析时间点唯一差异表达的LncRNAs(DElncRNAs)的功能富集性。结果表明，776个DElncRNAs是D4所特有的，其功能主要集中在伤口愈合、磷酸酶结合和MAPK信号通路上；317个DElncRNAs是D7所特有的，其功能主要集中在离子跨膜转运蛋白通道活性上；这两天共有579个DElncRNAs，其功能主要集中在轴突形成、PI3K-Akt信号通路和细胞周期等方面。此外，在功能或途径上构建了lncRNA-mRNA相互作用网络，富集率较高。最终筛选出轴突形成相互作用网络中的3个mRNAs和4个lncRNAs，并通过RT-qPCR验证其表达水平。本研究初步揭示了PNI后不同时间点LncRNAs的调控作用，为PNI的基础研究和临床治疗提供了潜在靶点。
The regulatory role of lncRNAs in the early stage post peripheral nerve injury (PNI) is not yet clear. In this study, next-generation sequencing was used to perform deep sequencing on normal sciatic nerves (control) and lesional tissues derived on the 4th (D4) and 7th days (D7) after sciatic nerve injury in rats. Time-point unique differentially expressed lncRNAs (DElncRNAs) were analyzed for functional enrichment. The results showed that 776 DElncRNAs were unique to D4, and their functions were mainly enriched in wound healing, phosphatase binding and MAPK signaling pathways; 317 DElncRNAs were unique to D7, and their functions were mainly enriched in ion transmembrane transporter channel activity; 579 DElncRNAs were shared by these two days, and their functions were mainly enriched in axongenesis, the PI3K-Akt signaling pathway and cell cycle. Furthermore, lncRNA-mRNA interaction networks were constructed in functions or pathways with a high enrichment rate. Finally, 3 mRNAs and 4 lncRNAs in the axongenesis interaction network were selected, and their expression levels were verified by RT-qPCR. This study preliminarily revealed the regulatory role of lncRNAs at different time points in the early stage post PNI, which provides potential targets for basic research and clinical treatment of PNI.