Astragaloside IV and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors
Astragaloside IV and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors
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DOI：
10.1023/a:1026233728375
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发表时间：
2003-11-01
影响因子：
2.7
通讯作者：
Branford-White, CJ
中科院分区：
文献类型：
--
作者：
Du, M;Wu, XJ;Branford-White, CJ
膜荚黄芪毛状根在30 L的生物反应器中生长20 d。来自30升气升式生物反应器的培养物得到11.5克/升干重和1.4毫克/克（-1）的黄芪甲苷IV，类似于来自250毫升和1升烧瓶的培养物，但大于来自10升生物反应器的产率（干重9.4克/升，黄芪甲苷IV 0.9毫克/克（-1））。多糖产量在不同的生物反应器之间是相似的（范围25-32 mg g-1）。细胞中的活性成分含量接近植物提取物的含量，表明毛状根的大规模培养是可行的。膜荚菇具有提供植物作物的替代品而不损害产量或药理学潜力的潜力。
Hairy roots of Astragalus membranaceus were grown in bioreactors up to 30 l for 20 d. Cultures from a 30 l airlift bioreactor gave 11.5 g l dry wt with 1.4 mg g(-1) astragaloside IV, similar to cultures from 250 ml and 1 l flasks, but greater than yields from a 10 l bioreactor (dry wt 9.4 g l(-1), astragaloside IV 0.9 mg g(-1)). Polysaccharide yields were similar amongst the different bioreactors (range 25-32 mg g-1). The active constituent content of the cells approached that of plant extracts, indicating that large scale hairy root cultures of A. membranaceus has the potential to provide an alternative to plant crops without compromising yield or pharmacological potential.