Expression of survivin in normal, hyperplastic, and neoplastic colonic mucosa
Expression of survivin in normal, hyperplastic, and neoplastic colonic mucosa
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DOI：
10.1053/hupa.2001.21897
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Shroyer, KR
中科院分区：
文献类型：
--
作者：
Gianani, R;Jarboe, E;Shroyer, KR
细胞凋亡的调节可能对结肠癌的发病机制和进展产生深远的影响。 Survivin 是凋亡抑制基因家族的成员，已在胎儿组织和多种人类恶性肿瘤中检测到。在当前的研究中，我们通过免疫组织化学方法研究了结肠良性、增生性、癌前和恶性病变中存活蛋白的表达。在正常结肠粘膜（20/20）、增生性息肉（20/20）、腺瘤性息肉（20/20）以及高分化和中分化结肠腺癌（20/20）的所有病例中均检测到存活蛋白。在正常结肠粘膜中，存活蛋白的表达主要局限于结肠隐窝的基底部。在增生性息肉中，所有上皮细胞都显示出均匀强烈的生存素染色。相比之下，腺瘤和腺癌表现出异质染色模式，细胞间、腺体间和生存素染色强度存在区域差异。与正常结肠粘膜中染色的基础优势相反，在增生性息肉、腺瘤性息肉和腺癌的管腔表面存在大量存活蛋白阳性细胞。总之，生存素的表达不是结肠腺癌的特异性标志物，但在非肿瘤性增殖性病变和正常结肠粘膜中确实显示出特征性和可重复的表达模式。版权所有 (C) 2001 W.B.桑德斯公司。
The regulation of apoptotic cell death may have a profound effect on the pathogenesis and progression of colon cancer. Survivin, a member of the inhibitor of apoptosis gene family, has been detected in fetal tissue and in a variety of human malignancies. In the current study, we investigated survivin expression by an immunohistochemical approach in benign, hyperplastic, premalignant, and malignant lesions of the colon. Survivin was detected in all cases of normal colonic mucosa (20/20), hyperplastic polyps (20/20), adenomatous polyps (20/20), and in both well differentiated and moderately differentiated colonic adenocarcinomas (20/20). In the normal colonic mucosa, survivin expression was mostly restricted to the base of the colonic crypts. All epithelial cells showed uniformly intense staining for survivin in hyperplastic polyps. By contrast, adenomas and adenocarcinomas showed a heterogeneous staining pattern with cell-to-cell, gland-to-gland, and regional variability in the intensity of survivin staining. In contrast to the basal preponderance of staining in normal colonic mucosa, numerous survivin positive cells were present at the luminal surface of hyperplastic polyps, adenomatous polyps, and adenocarcinomas. In conclusion, the expression of survivin is not a specific marker of adenocarcinoma of the colon but does show characteristic and reproducible patterns of expression in non-neoplastic proliferative lesions and in normal colonic mucosa. Copyright (C) 2001 by W.B. Saunders Company.