Aminoglycoside-induced damage in the statocyst of the longfin inshore squid, Doryteuthis pealeii.
Aminoglycoside-induced damage in the statocyst of the longfin inshore squid, Doryteuthis pealeii.
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氨基糖苷引起的长鳍近海鱿鱼 Doryteuthis pealeii 的静囊损伤。
DOI：
10.1086/bblv227n1p51
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Ketten,DarleneR
中科院分区：
文献类型：
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作者：
Scharr,AlexandraL;Mooney,TAran;Schweizer,FelixE;Ketten,DarleneR
鱿鱼是海洋生物量的重要组成部分，也是实验神经生理学中一种长期确立的模式生物。鱿鱼平衡囊感知线性和角加速度，是介导鱿鱼听觉反应的最佳候选者，但其生理和形态学研究很少。平衡囊含有机械敏感的毛细胞，类似于其他动物前庭和听觉系统中的毛细胞。我们研究了鱿鱼平衡囊毛细胞是否对氨基糖苷类敏感，氨基糖苷类是一组在鱼类，鸟类和哺乳动物中具有耳毒性的抗生素。为了评估氨基糖苷类药物引起的损伤，我们使用免疫荧光方法成像的主要细胞类型的平衡囊的长鳍鱿鱼（Doryteuthis pealeii）。将浓度范围为0.05至3.0 mmol l-1的缓冲盐水溶液或新霉素注射到活的麻醉鱿鱼的静囊中。处理后5 h检查主静止斑的平衡囊毛细胞。抗乙酰化微管蛋白染色显示，盐水注射和非注射平衡囊的毛细胞之间没有形态学差异。在注射氨基糖苷类的平衡囊中，主静止斑的毛细胞束缺失或受损，损伤量呈剂量依赖性。缺失毛细胞的比例没有以与受损细胞相同的速率增加，这表明新霉素治疗以非致死方式影响毛细胞。这些实验为鱿鱼毛细胞的成像提供了可靠的方法。此外，氨基糖苷类可用于诱导鱿鱼平衡囊的初级感觉区中的毛细胞损伤。这些结果支持了对鱿鱼听力和平衡损失的进一步研究。
Squid are a significant component of the marine biomass and are a long-established model organism in experimental neurophysiology. The squid statocyst senses linear and angular acceleration and is the best candidate for mediating squid auditory responses, but its physiology and morphology are rarely studied. The statocyst contains mechano-sensitive hair cells that resemble hair cells in the vestibular and auditory systems of other animals. We examined whether squid statocyst hair cells are sensitive to aminoglycosides, a group of antibiotics that are ototoxic in fish, birds, and mammals. To assess aminoglycoside-induced damage, we used immunofluorescent methods to image the major cell types in the statocyst of longfin squid (Doryteuthis pealeii). Statocysts of live, anesthetized squid were injected with either a buffered saline solution or neomycin at concentrations ranging from 0.05 to 3.0 mmol l−1. The statocyst hair cells of the macula statica princeps were examined 5 h post-treatment. Anti-acetylated tubulin staining showed no morphological differences between the hair cells of saline-injected and non-injected statocysts. The hair cell bundles of the macula statica princeps in aminoglycoside-injected statocysts were either missing or damaged, with the amount of damage being dose-dependent. The proportion of missing hair cells did not increase at the same rate as damaged cells, suggesting that neomycin treatment affects hair cells in a nonlethal manner. These experiments provide a reliable method for imaging squid hair cells. Further, aminoglycosides can be used to induce hair cell damage in a primary sensory area of the statocyst of squid. Such results support further studies on loss of hearing and balance in squid.