THE INDUCTION OF ODONTOGENESIS IN NONDENTAL MESENCHYME COMBINED WITH EARLY MURINE MANDIBULAR ARCH EPITHELIUM
THE INDUCTION OF ODONTOGENESIS IN NONDENTAL MESENCHYME COMBINED WITH EARLY MURINE MANDIBULAR ARCH EPITHELIUM
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DOI：
10.1016/0003-9969(87)90055-0
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发表时间：
1987-01-01
影响因子：
3
通讯作者：
KOLLAR, EJ
中科院分区：
文献类型：
--
作者：
MINA, M;KOLLAR, EJ
从胎龄9~13天的小鼠胚胎中解剖出第一、二鳃弓。酶消化后分离上皮和间充质成分。扫描电子显微镜在12天前未发现沿牙弓的牙板，之后在切牙和磨牙区局部形成牙板。间隔区无上皮样向下生长。下颌弓和第二鳃弓组织的异型重组表明，12天前的早期下颌弓上皮细胞具有成牙潜能，并能在第二牙弓的非牙源性神经冠间充质细胞中诱导形成牙乳头。然而，下颌间充质必须与下颌上皮细胞相互作用，才能在非牙源性上皮细胞中诱导成牙。
First and second branchial arches were dissected from mouse embryos of 9-13 days gestational age. The epithelial and mesenchymal components were separated after enzymic digestion. Scanning electron microscopy did not reveal a dental lamina along the dental arches before day 12, after which the lamina was formed locally in the incisor and molar regions. There was no epithelial down-growth in the diastema region. Heterotypic recombination of mandibular arch and second branchial arch tissues showed that early mandibular arch epithelia, before day 12, have odontogenic potential and can elicit the formation of a dental papilla in non-odontogenic, neural-crest-derived mesenchymal cells of the second arch. However, the mandibular mesenchyme must interact with mandibular epithelium in order to have the competence to induce teeth in non-odontogenic epithelium.