An ultra-sensitive dual-mode imaging system using metal-enhanced fluorescence in solid phantoms.
An ultra-sensitive dual-mode imaging system using metal-enhanced fluorescence in solid phantoms.
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DOI：
10.1007/s12274-015-0891-y
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发表时间：
2015-12-01
期刊：
影响因子：
9.9
通讯作者：
Ray K
中科院分区：
文献类型：
--
作者：
Barnoy EA;Fixler D;Popovtzer R;Nayhoz T;Ray K
在这项研究中，我们开发了一种高灵敏度的双峰成像系统，设计用于金纳米粒子（GNPs）在固体幻影中共轭到各种荧光团。该系统包括用于表面成像的荧光寿命成像显微镜（FLIM），用于深层组织成像（高达1cm）的扩散反射显微镜（DR）和金属增强荧光（MEF）。我们检测到金纳米球（GNS）和金纳米棒（GNRs）与荧光素结合的荧光强度（FI）猝灭，荧光素的激发峰波长比两种GNPs的表面等离子体共振（SPR）波长短，而与罗丹明B和硫代丹明B结合的荧光强度（FI）增强，两者的激发峰都在GNPs的SPR中。增强的FI在溶液中被检测到，以及在固体幻影从FLIM测量。DR测量通过记录与GNR吸收相对应的波长的光散射下降率来检测固体幻影中GNR的存在。随着MEF的加入，这种有前途的双峰成像技术能够实现高效、灵敏的分子和功能成像。
In this study we developed a highly sensitive dual modal imaging system designed for gold nanoparticles (GNPs) conjugated to various fluorophores in solid phantoms. The system consists of fluorescence lifetime imaging microscopy (FLIM) for surface imaging, diffusion reflection (DR) for deep tissue imaging (up to 1cm), and metal enhanced fluorescence (MEF). We detected quenching in fluorescent intensity (FI) for the conjugation of gold nanospheres (GNS) as well as gold nanorods (GNRs) to Fluorescein, which has an excitation peak at a wavelength shorter than the surface plasmon resonance (SPR) of both types of GNPs, and enhanced FI in conjugation to Rhodamine B and Sulforhodamine B, both with excitation peaks in the GNPs' SPR. The enhanced FI was detected in solution as well as in solid phantoms from FLIM measurements. DR measurements detected GNR presence within the solid phantoms by recording dropped rates of light scattering using wavelengths corresponding to the GNRs' absorption. With the inclusion of MEF, this promising dual modal imaging technique enables efficient and sensitive molecular and functional imaging.