Effect of ultrasonically nebulized distilled water on airway epithelial cell swelling in guinea pigs
Effect of ultrasonically nebulized distilled water on airway epithelial cell swelling in guinea pigs
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DOI：
10.1152/jappl.1999.86.5.1505
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发表时间：
1999-05-01
影响因子：
3.3
通讯作者：
Morikawa, A
中科院分区：
文献类型：
--
作者：
Mochizuki, H;Ohki, Y;Morikawa, A
为了研究超声雾化蒸馏水引起的气道狭窄的发病机制，我们研究了气道炎症动物模型中蒸馏水吸入挑战期间气道上皮细胞的作用。将豚鼠分为四组：1）假手术/盐水（S/S）组：假手术臭氧，然后吸入盐水； 2)假/水(S/W)组：假臭氧，然后吸入水； 3) 臭氧/盐水（O/S）组：先吸入臭氧，然后吸入盐水； 4) 臭氧/水（O/W）组：先吸入臭氧，然后吸入水。在以相同流速暴露于3.0 ppm的臭氧或空气2小时后，将豚鼠麻醉并进行气管切开术，然后测量肺阻力（RL）。为了进行形态测定评估，将组织用甲醛固定，用苏木精和曙红染色，并切成横切片。气道尺寸直接测量或根据内周长、外周长和气道壁面积计算。假手术组与臭氧组吸入蒸馏水前的RL值无统计学差异。 S/W组和O/W组吸入蒸馏水10分钟后RL显着增加。 S/W组上皮细胞肿胀，细胞间隙增宽，上皮壁厚度明显增加，但未见明显炎症细胞浸润。在O/S组中，上皮细胞显示炎症细胞浸润，但细胞体积没有变化。在O/W组中，与S/W组相比，上皮表现出浸润并且上皮壁厚度有更大的增加。这些结果表明，豚鼠吸入蒸馏水引起的气道上皮细胞肿胀随着 RL 的增加而增加，并且气道炎症可能会加速这种反应。
To investigate the pathogenesis of ultrasonically nebulized distilled water-induced airway narrowing, we studied the role of airway epithelial cells during a distilled water-inhalation challenge in an animal model of airway inflammation. Guinea pigs were divided into four groups: 1) a sham/saline (S/S) group: sham ozone followed by saline inhalation; 2) a sham/water (S/W) group: sham ozone followed by water inhalation; 3) an ozone/saline (O/S) group: ozone followed by saline inhalation; and 4) an ozone/water (O/W) group: ozone followed by water inhalation. After exposure to either 3.0 parts/million ozone or air at the same flow rate for 2 h, guinea pigs were anesthetized and tracheostomized, and then lung resistance (RL) was measured. For morphometric assessment, tissues were fixed with formaldehyde, stained with hematoxylin and eosin, and cut into transverse sections. Airway dimensions were either measured directly or calculated from the internal perimeter, the external perimeter, and airway wall area. There were no statistical differences in the values of RL before distilled water inhalation between the sham groups and the ozone groups. RL increased significantly after 10 min of distilled water inhalation in both the S/W group and the O/W group. In the S/W group, epithelial cells were swollen, and intercellular spaces were wider, resulting in significant increase in epithelial wall thickness, but there was no significant infiltration by inflammatory cells. In the O/S group, the epithelium showed infiltration by inflammatory cells without change in cell volume. In the O/W group, the epithelium showed both infiltration and a greater increase in epithelial wall thickness compared with the S/W group. These results suggest that airway epithelial cell swelling, induced by inhaled distilled water, increases with RL in guinea pigs and that this reaction may be accelerated by airway inflammation.