DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli
DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli
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DOI：
10.1073/pnas.220414297
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Yanofsky, C
中科院分区：
文献类型：
--
作者：
Khodursky, AB;Peter, BJ;Yanofsky, C
我们研究了色氨酸代谢的各种扰动引起的大肠杆菌中mRNA丰度的全球变化。为此，我们打印了包含95%注释的E.ColiORF的DNA微阵列。我们确定了主要由色氨酸抑制物活性决定的表达谱。只有三个操纵子，Trp，MTR和AROH，表现出与这个图谱一致的明显的表达变化。我们观察到的FRP操纵子的五个基因的mRNA水平的定量变化在因子2内是一致的，与基于已建立的Trp蛋白水平的预期一致。几个已知受TyrR蛋白调控的操纵子，aroF-tyra、arol、arop和arg。在添加色氨酸后表达下调。TyrR可以被以下三种芳香氨基酸中的任何一种激活。只有一个操纵子。TNAAB可被培养液中色氨酸显著激活。我们发现了色氨酸代谢变化对细胞内信使核糖核酸库的大量可能的间接影响，其中最突出的是精氨酸生物合成操纵子对色氨酸饥饿的敏感性。
We investigated the global changes in mRNA abundance in Escherichia coli elicited by various perturbations of tryptophan metabolism. To do so we printed DNA microarrays containing 95% of all annotated E. coli ORFs. We determined the expression profile that is predominantly dictated by the activity of the tryptophan repressor. Only three operons, trp, mtr, and aroH, exhibited appreciable expression changes consistent with this profile. The quantitative changes we observed in mRNA levels for the five genes of the frp operon were consistent within a factor of 2, with expectations based on established Trp protein levels. Several operons known to be regulated by the TyrR protein, aroF-tyrA, aroL, aroP, and aroG. were down-regulated on addition of tryptophan. TyrR can be activated by any one of the:three aromatic amino acids. Only one operon. tnaAB, was significantly activated by the presence of tryptophan in the medium. We uncovered a plethora of likely indirect effects of changes in tryptophan metabolism on intracellular mRNA pools, most prominent of which was the sensitivity of arginine biosynthetic operons to tryptophan starvation.