Characterization of proteases in the skin mucus of Atlantic salmon (Salmo salar) infected with the salmon louse (Lepeophtheirus salmonis) and in whole-body louse homogenate
Characterization of proteases in the skin mucus of Atlantic salmon (Salmo salar) infected with the salmon louse (Lepeophtheirus salmonis) and in whole-body louse homogenate
复制标题
DOI：
10.1645/0022-3395(2000)086
复制
发表时间：
2000-12-01
影响因子：
1.3
通讯作者：
Ross, NW
中科院分区：
文献类型：
--
作者：
Firth, KJ;Johnson, SC;Ross, NW
作为鲑鱼虱Lepeophtheirus salmonis和大西洋鲑鱼萨尔莫salar之间的生化相互作用的调查的一部分，我们表征了未感染的大西洋鲑鱼和感染Lepeophtheirus的大西洋鲑鱼的皮肤粘液中的蛋白酶活性。salmonis和L. salmonis全身匀浆中。酶谱分析显示，从感染鲑鱼粘液含有一系列的低分子量（17-22 kDa）丝氨酸蛋白酶，不存在于未感染鲑鱼粘液。基于分子量，抑制研究，和亲和层析，该系列蛋白酶被确定为胰蛋白酶样。在L. salmonis匀浆中以及来自未感染的大西洋鲑鱼的粘液中，与活的L.鲑鱼大西洋鲑鱼胰蛋白酶的抗体不能识别未感染或感染鲑鱼粘液中的任何蛋白酶，也不能识别L。鲑鱼匀浆总的来说，这些发现表明，在感染的大西洋鲑鱼粘液中存在的胰蛋白酶样蛋白酶是由L。作为鲑鱼虱Lepeophtheirus salmonis和大西洋鲑鱼萨尔莫salar之间的生化相互作用的调查的一部分，我们表征了未感染的大西洋鲑鱼和感染Lepeophtheirus的大西洋鲑鱼的皮肤粘液中的蛋白酶活性。salmonis和L. salmonis全身匀浆中。酶谱分析显示，从感染鲑鱼粘液含有一系列的低分子量（17-22 kDa）丝氨酸蛋白酶，不存在于未感染鲑鱼粘液。基于分子量，抑制研究，和亲和层析，该系列蛋白酶被确定为胰蛋白酶样。在L. salmonis匀浆中以及来自未感染的大西洋鲑鱼的粘液中，与活的L.鲑鱼大西洋鲑鱼胰蛋白酶的抗体不能识别未感染或感染鲑鱼粘液中的任何蛋白酶，也不能识别L。鲑鱼匀浆总的来说，这些发现表明，在感染的大西洋鲑鱼粘液中存在的胰蛋白酶样蛋白酶是由L。鲑鱼，可能有助于进食和逃避宿主的免疫反应。
AS part of an investigation of the biochemical interactions between the salmon louse Lepeophtheirus salmonis and Atlantic salmon Salmo salar, we characterized protease activity in the skin mucus of noninfected Atlantic salmon and Atlantic salmon infected with L. salmonis and in an L, salmonis whole-body homogenate. Zymography revealed that mucus from infected salmon contained a series of low-molecular-mass (17-22 kDa) serine proteases that were not present in the mucus of noninfected salmon. Based on molecular mass, inhibition studies, and affinity chromatography, the series of proteases was identified as being trypsin-like. Similar proteases were observed in the L. salmonis homogenate and in mucus from noninfected Atlantic salmon following a 1-hr incubation with live L. salmonis. An antibody raised against Atlantic salmon trypsin failed to recognize any proteases in the mucus of noninfected salmon or infected salmon or in the L. salmonis homogenate. Collectively, these findings suggest that the trypsin-like proteases present in the mucus of infected Atlantic salmon were produced by L. salmonis, possibly to aid in feeding and evasion of host immune responses.As part of an investigation of the biochemical interactions between the salmon louse Lepeophtheirus salmonis and Atlantic salmon Salmo salar, we characterized protease activity in the skin mucus of noninfected Atlantic salmon and Atlantic salmon infected with L. salmonis and in an L, salmonis whole-body homogenate. Zymography revealed that mucus from infected salmon contained a series of low-molecular-mass (17-22 kDa) serine proteases that were not present in the mucus of noninfected salmon. Based on molecular mass, inhibition studies, and affinity chromatography, the series of proteases was identified as being trypsin-like. Similar proteases were observed in the L. salmonis homogenate and in mucus from noninfected Atlantic salmon following a 1-hr incubation with live L. salmonis. An antibody raised against Atlantic salmon trypsin failed to recognize any proteases in the mucus of noninfected salmon or infected salmon or in the L. salmonis homogenate. Collectively, these findings suggest that the trypsin-like proteases present in the mucus of infected Atlantic salmon were produced by L. salmonis, possibly to aid in feeding and evasion of host immune responses.