Effect of prior experimental human enteropathogenic Escherichia coli infection on illness following homologous and heterologous rechallenge
Effect of prior experimental human enteropathogenic Escherichia coli infection on illness following homologous and heterologous rechallenge
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DOI：
10.1128/iai.66.1.52-58.1998
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发表时间：
1998-01-01
影响因子：
3.1
通讯作者：
Levine, MM
中科院分区：
文献类型：
--
作者：
Donnenberg, MS;Tacket, CO;Levine, MM
研究人员对成年志愿者进行了两项研究，以确定德里耶肠致病性大肠杆菌（EPEC）感染是否会对再攻击产生保护性免疫。在第一项研究中，一个没有经验的对照组和之前摄入过O55:H6菌株的志愿者被喂食了O127:H6菌株。在第二项研究中，对照组和先前摄入过O127:H6菌株或该菌株的等基因eae缺失突变体的志愿者接受了同源野生型菌株的攻击。两项研究均未发现既往感染对腹泻发生率的显著影响。然而，在同源再攻毒研究中，先前用野生型菌株攻毒的组的疾病明显较轻。疾病严重程度与攻毒前血清抗O127脂多糖免疫球蛋白G水平呈负相关。这些研究表明，先前的EPEC感染可以在同源攻击下降低疾病的严重程度。进一步的研究可能需要开发新的模型系统。
Two studies of adult volunteers were performed to determine whether Drier enteropathogenic Escherichia coli (EPEC) infection confers protective immunity against rechallenge. In the first study, a naive control group and volunteers who had previously ingested an O55:H6 strain were fed an O127:H6 strain. In the second study, a control group and volunteers who had previously ingested either the O127:H6 strain or an isogenic eae deletion mutant of that strain were challenged with the homologous wild-type strain. There was no significant effect of prior infection on the incidence of diarrhea in either study. However, in the homologous-rechallenge study, disease was significantly milder in the group previously challenged with the wild-type strain. Disease severity was inversely correlated with the level of prechallenge serum immunoglobulin G against the O127 lipopolysaccharide. These studies indicate that prior EPEC infection can reduce disease severity upon homologous challenge. Further studies may require the development of new model systems.