Heterogeneity of microvascular pericytes for smooth muscle type alpha-actin.
Heterogeneity of microvascular pericytes for smooth muscle type alpha-actin.
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DOI：
10.1083/jcb.113.1.147
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发表时间：
1991-04
影响因子：
7.8
通讯作者：
Drenckhahn, D
中科院分区：
文献类型：
--
作者：
Nehls, V;Drenckhahn, D
微血管周细胞被认为参与了多种功能，如调节毛细血管血流和内皮细胞增殖。由于周细胞代表着一种形态不同的细胞群体，从圆形光滑的肌肉样形态到细长的成纤维细胞样形态，因此可能通过不同的周细胞亚群来完成血流的调节(通过收缩)和内皮增殖的控制(以及其他代谢功能)。在目前的研究中，我们通过使用两种新的技术方法在分子水平上为周细胞的异质性提供了证据。这些是(A)对大鼠肠系膜和牛视网膜微血管床的整体进行免疫染色，以及(B)对显微解剖的视网膜微血管进行免疫印迹研究。我们发现，真正的毛细血管周细胞(中毛细血管)明显缺乏α-肌动蛋白的平滑肌动蛋白亚型，而毛细血管前段和后微血管段的过渡性周细胞确实表达这种亚型。因此，毛细血管血流的调节可能是通过与平滑肌相关的毛细血管前周细胞和毛细血管后周细胞完成的，而真正毛细血管的非肌肉周细胞可能在其他功能中发挥作用。
Microvascular pericytes are believed to be involved in various functions such as regulation of capillary blood flow and endothelial proliferation. Since pericytes represent a morphologically heterogeneous cell population ranging from circular smooth musclelike to elongated fibroblast-like morphology it is possible that regulation of blood flow (via contractility) and control of endothelial proliferation (as well as other metabolic functions) may be accomplished by different subsets of pericytes. In the present study we provide evidence for heterogeneity of pericytes at the molecular level by using two novel technical approaches. These are (a) immunostaining of whole mounts of the microvascular beds of the rat mesentery and bovine retina and (b) immunoblotting studies of microdissected retinal microvessels. We show that pericytes of true capillaries (midcapillaries) apparently lack the smooth muscle isoform of alpha- actin whereas transitional pericytes of pre- and postcapillary microvascular segments do express this isoform. Thus, regulation of capillary blood flow may be accomplished by the smooth muscle-related pre- and postcapillary pericytes whereas the nonmuscle pericytes of true capillaries may play a role in other functions.