Natural malaria infection in Anopheles gambiae is regulated by a single genomic control region
Natural malaria infection in Anopheles gambiae is regulated by a single genomic control region
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DOI：
10.1126/science.1124153
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发表时间：
2006-04-28
期刊：
影响因子：
56.9
通讯作者：
Vernick, KD
中科院分区：
文献类型：
--
作者：
Riehle, MM;Markianos, K;Vernick, KD
我们调查了冈比亚按蚊种群在西非疟疾传播区的自然发生的遗传位点，控制蚊子感染人类疟疾寄生虫，恶性疟原虫。最强的疟原虫抗性位点簇在染色体2L的一个小区域，每个位点解释了独立谱系中至少89%的无寄生虫蚊子。这些聚集的基因座共同形成了一个基因组抗疟原虫岛，解释了自然界中蚊子感染疟原虫的大部分遗传变异。在该染色体区域的候选基因中，RNA干扰敲低测定证实了按蚊疟原虫应答性富含亮氨酸重复序列1（APL1）在疟原虫抗性中的作用，APL1编码富含亮氨酸的重复序列蛋白，其类似于植物和哺乳动物中天然病原体抗性机制中所涉及的分子。
We surveyed an Anopheles gambiae population in a West African malaria transmission zone for naturally occurring genetic loci that control mosquito infection with the human malaria parasite, Plasmodium falciparum. The strongest Plasmodium resistance loci cluster in a small region of chromosome 2L and each locus explains at least 89% of parasite-free mosquitoes in independent pedigrees. Together, the clustered loci form a genomic Plasmodium-resistance island that explains most of the genetic variation for malaria parasite infection of mosquitoes in nature. Among the candidate genes in this chromosome region, RNA interference knockdown assays confirm a role in Plasmodium resistance for Anopheles Plasmodium-responsive leucine-rich repeat 1 (APL1), encoding a leucine-rich repeat protein that is similar to molecules involved in natural pathogen resistance mechanisms in plants and mammals.