Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides.
Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides.
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DOI：
10.1021/acsnano.5b02374
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发表时间：
2015-09-22
期刊：
影响因子：
17.1
通讯作者：
Hammond PT
中科院分区：
文献类型：
--
作者：
Hsu BB;Conway W;Tschabrunn CM;Mehta M;Perez-Cuevas MB;Zhang S;Hammond PT
创伤伤口不受控制地流血是军事冲突、事故、灾害和犯罪造成死亡的一个主要因素。自组装肽纳米纤维已显示出上级止血活性，并且在此，我们通过可视化基于纳米纤维的凝块的形成来阐明其机制，所述凝块聚集具有与基于纤维蛋白的凝块相似的形态的血液成分。此外，为了增强其在伤口上的直接应用，我们在用于伤口敷料的常用材料纱布和明胶海绵上开发了逐层组装的薄膜涂层。我们发现这些纳米纤维在生理条件下水合后会分解，并在血液中产生基于纳米纤维的凝块。在暴露于一系列苛刻的温度条件（−80至60 °C）一周甚至在60 °C下5个月后，这些止血绷带仍然能够释放活性纳米纤维。此外，发现与普通纱布相比，从纱布绷带中应用这些基于纳米纤维的膜可加速猪皮肤伤口的止血。热稳定性与自组装肽的有效止血活性、生物相容性、生物降解性和低生产成本相结合，使其成为廉价而有效的止血绷带的有希望的方法。
Uncontrolled bleeding from traumatic wounds is a major factor in deaths resulting from military conflict, accidents, disasters and crime. Self-assembling peptide nanofibers have shown superior hemostatic activity, and herein, we elucidate their mechanism by visualizing the formation of nanofiber-based clots that aggregate blood components with a similar morphology to fibrin-based clots. Furthermore, to enhance its direct application to a wound, we developed layer-by-layer assembled thin film coatings onto common materials used for wound dressings—gauze and gelatin sponges. We find these nanofibers elute upon hydration under physiological conditions and generate nanofiber-based clots with blood. After exposure to a range of harsh temperature conditions (−80 to 60 °C) for a week and even 5 months at 60 °C, these hemostatic bandages remain capable of releasing active nanofibers. In addition, the application of these nanofiber-based films from gauze bandages was found to accelerate hemostasis in porcine skin wounds as compared to plain gauze. The thermal robustness, in combination with the self-assembling peptide’s potent hemostatic activity, biocompatibility, biodegradability, and low cost of production, makes this a promising approach for a cheap yet effective hemostatic bandage.