Changing patterns of transcriptional and post-transcriptional control of liver-specific gene expression during rat development.
Changing patterns of transcriptional and post-transcriptional control of liver-specific gene expression during rat development.
复制标题
大鼠发育过程中肝脏特异性基因表达的转录和转录后控制模式的变化。
DOI：
10.1101/gad.1.10.1172
复制
发表时间：
1987
影响因子：
10.5
通讯作者：
Shafritz,DA
中科院分区：
文献类型：
--
作者：
Panduro,A;Shalaby,F;Shafritz,DA
编码肝脏中独特或组织特异性（分化）功能的基因在发育过程中的不同时间被诱导。人们普遍认为，转录控制代表了调节这些基因表达的主导机制。我们已经确定了一系列在大鼠肝脏中特异或优先表达的基因的相对转录速率和mRNA稳态水平，并找到了转录控制的例子（白蛋白、甲胎蛋白、α 1-抗胰蛋白酶、酪氨酸氨基转移酶、转铁蛋白和细胞色素P450、TF-1）和转录后控制（α 1-酸性糖蛋白、载脂蛋白A-1和E、苹果酸酶和ATP柠檬酸裂解酶），以及“混合”调节（配体蛋白和细胞色素P450，R17）。已经确定的例子，其中的主要模式，用于调节表达的优先表达的基因的变化，从转录转录后在不同阶段的肝脏发育和多基因家族（细胞色素P450和载脂蛋白基因）的一些成员也显示出独立的，有时对比的调节模式。因此，看来肝脏中特定基因表达的调节是一个动态过程，比迄今为止所怀疑的复杂得多，并且转录后调节的贡献大得多，这说明了代表肝脏主要功能的基因表达的变化。
Genes coding for unique or tissue-specific (differentiated) functions in the liver are induced at different times during development. It has generally been felt that transcriptional control represents the dominant mechanism for regulating expression of these genes. We have determined the relative transcription rates and mRNA steady-state levels for a series of genes specifically or preferentially expressed in rat liver and find examples of transcriptional control (albumin, alpha-fetoprotein, alpha 1-antitrypsin, tyrosine aminotransferase, transferrin, and cytochrome P450, TF-1) and post-transcriptional control (alpha 1-acid glycoprotein, apolipoproteins A-1 and E, malic enzyme, and ATP citrate lyase), as well as "mixed" regulation (ligandin and cytochrome P450, R17). Examples have been identified in which the predominant mode for regulating expression of preferentially expressed genes changes from transcriptional to post-transcriptional at different stages of liver development and some members of multigene families (cytochrome P450s and apolipoprotein genes) also show independent and sometimes contrasting modes of regulation. Therefore, it appears that regulation of specific gene expression in the liver is a dynamic process, far more complex than heretofore suspected, and a much greater contribution of post-transcriptional regulation accounts for changes in expression of genes representing major functions of the liver.