Xiaoyaosan improves depressive-like behavior in rats with chronic immobilization stress through modulation of the gut microbiota
Xiaoyaosan improves depressive-like behavior in rats with chronic immobilization stress through modulation of the gut microbiota
复制标题
逍遥散通过调节肠道菌群改善慢性固定应激大鼠的抑郁样行为
DOI：
10.1016/j.biopha.2019.108621
复制
发表时间：
2019-04-01
影响因子：
7.5
通讯作者：
Chen, Jia-Xu
中科院分区：
文献类型：
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作者：
Zhu, Hui-Zheng;Liang, Yu-Dan;Chen, Jia-Xu
抑郁症已成为全球残疾的主要原因，也是中国日益严重的公共卫生问题。此外，肠道菌群可能与抑郁症有关。本研究探讨逍遥散通过调节肠道菌群抗抑郁行为的作用。健康雄性SD大鼠52只，随机分为4组，即对照组、模型组、XY组和氟西汀组。后3组大鼠接受21d慢性束缚应激，建立应激抑郁模型。XYS组和氟西汀组分别给予XYS和氟西汀灌胃。21d后观察大鼠行为学变化。用16S rDNA高通量方法对粪便标本进行测序，以检测肠道菌群结构和变化。在不同的群体中，没有观察到阿尔法多样性的差异。在门水平上，XY调控类杆菌、变形杆菌、假丝酵母、氯杆菌和扁平霉菌的丰度。在属水平上，XY降低了普氏弧菌_Ga6A1_群、普氏弧菌_UCG-001和脱硫弧菌的丰度。相反，它增加了反刍球菌科植物的丰度，以改善抑郁样行为。参与这一过程的机制可能与短链脂肪酸、脂多糖和肠道炎症有关。
Depression has become the leading cause of disability worldwide and a growing public health problem in China. In addition, intestinal flora may be associated with depression. This study investigated the effect of the decoction Xiaoyaosan (XYS) against depressive behavior through the regulation of intestinal flora. Fifty-two healthy male Sprague-Dawley rats were randomly divided into four groups (i.e., control, model, XYS, and fluoxetine). The latter three groups were subjected to 21 days of chronic restraint stress to produce the stress depression model. Rats in the XYS and fluoxetine groups received intragastric administration of XYS and fluoxetine, respectively. The behavioral changes of the rats were observed after 21 days. Stool specimens were sequenced using the 16S rDNA high-throughput method to detect the structure and changes in intestinal flora. There was no difference observed in alpha diversity among the groups. At the phylum level, XYS regulated the abundance of Bacteroidetes, Proteobacteria, Firmicutes, Chloroflexi, and Planctomycetes. At the genus level, XYS reduced the abundance of the Prevotellaceae_Ga6A1_group, Prevotellaceae_UCG-001, and Desulfovibrio. On the contrary, it increased the abundance of the Ruminococcaceae family to improve depression-like behavior. The mechanism involved in this process may be related to short-chain fatty acids, lipopolysaccharides, and intestinal inflammation.