Reinforcing the LINC complex connection to actin filaments: the role of FHOD1 in TAN line formation and nuclear movement
Reinforcing the LINC complex connection to actin filaments: the role of FHOD1 in TAN line formation and nuclear movement
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DOI：
10.1080/15384101.2015.1053665
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发表时间：
2015-07-18
期刊：
影响因子：
4.3
通讯作者：
Gundersen, Gregg G.
中科院分区：
文献类型：
--
作者：
Antoku, Susumu;Zhu, Ruijun;Gundersen, Gregg G.
定位细胞核对于许多细胞过程包括细胞分裂、迁移和分化是至关重要的。核骨架和细胞骨架（LINC）复合物的连接体跨越核膜内外，并且已经成为连接核和细胞骨架以进行运动和定位的主要因素。最近，我们发现透明的肌动蛋白家族成员FHOD 1与LINC复合物组分nesprin-2 giant（nesprin-2G）相互作用，并且这种相互作用在成纤维细胞中细胞极化期间跨膜肌动蛋白依赖性核（TAN）系和核运动的形成中起重要作用。我们发现，FHOD 1通过提供nesprin-2G和肌动蛋白索之间的第二个相互作用位点，加强了nesprin-2G和向后移动的背侧肌动蛋白索之间的连接。这些结果表明，LINC复合物连接到肌动蛋白细胞骨架可以增强细胞质因子，并提出了一个新的模型TAN线的形成。我们讨论如何nesprin-2G-FHOD 1相互作用可能受到调节，其可能的功能意义的发展和疾病。
Positioning the nucleus is critical for many cellular processes including cell division, migration and differentiation. The linker of nucleoskeleton and cytoskeleton (LINC) complex spans the inner and outer nuclear membranes and has emerged as a major factor in connecting the nucleus to the cytoskeleton for movement and positioning. Recently, we discovered that the diaphanous formin family member FHOD1 interacts with the LINC complex component nesprin-2 giant (nesprin-2G) and that this interaction plays essential roles in the formation of transmembrane actin-dependent nuclear (TAN) lines and nuclear movement during cell polarization in fibroblasts. We found that FHOD1 strengthens the connection between nesprin-2G and rearward moving dorsal actin cables by providing a second site of interaction between nesprin-2G and the actin cable. These results indicate that the LINC complex connection to the actin cytoskeleton can be enhanced by cytoplasmic factors and suggest a new model for TAN line formation. We discuss how the nesprin-2G-FHOD1 interaction may be regulated and its possible functional significance for development and disease.