Oocyte size, a means to evaluate the gametogenic development of the Pacific oyster, Crassostrea gigas (Thunberg)
Oocyte size, a means to evaluate the gametogenic development of the Pacific oyster, Crassostrea gigas (Thunberg)
复制标题
卵母细胞大小，一种评估太平洋牡蛎配子发育的方法，Crassostrea gigas (Thunberg)
DOI：
10.1016/s0044-8486(00)00392-6
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
M. Pennec
中科院分区：
文献类型：
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作者：
F. Lango;J. Chávez‐Villalba;J. Cochard;M. Pennec
这项研究报告了对生活在法国大西洋海岸线上的三个种群的太平洋牡蛎（长牡蛎）卵母细胞直径进行38100次测量的结果。对1996年、1997年和1998年在布列斯特湾（Anse du Roz和Pointe du ch<e:1>角）和marennes - olsamuron采集的长尾螺卵母细胞直径的分析和性腺的组织学检查表明，长尾螺具有明显的季节性配子体发育模式。不同生殖阶段、生殖部位和生殖年龄的卵母细胞直径差异无统计学意义（*P>0.05）。考虑到该物种的定性和定量特征，我们提出了一个基于卵母细胞直径的生殖尺度，包括四个阶段：(1)“早期配子体”，卵母细胞直径平均为8.47±4.6 μm;(2)“生长期”，卵母细胞直径平均为21.4±8.4 μm;(3)“成熟期”，卵母细胞直径平均为36±4.4 μm;(4)“退化期”，卵母细胞直径平均为46±7.3 μm。这个尺度是由模态分布分析（用于拟合分布混合的交互式程序）和性腺特征的微观描述生成的。讨论了该尺度在巨螺繁殖研究中的应用。
This study reports on the results of 38,100 measurements of the oocyte diameters of the Pacific oyster, Crassostrea gigas, in three populations living along the French Atlantic shoreline. The analysis of oocytes' diameters and histological examinations of the gonads indicated that C. gigas individuals collected in 1996, 1997 and 1998 in the Bay of Brest (Anse du Roz and Pointe du Château) and Marennes-Oléron showed a clearly defined pattern of seasonal gametogenic development. Comparisons between reproductive stages, sites and years did not reveal significant differences in oocyte diameter (*P>0.05). Considerations from qualitative and quantitative characteristics of this species allowed us to propose a reproductive scale based on oocyte diameter and consisting of four stages: (1) “Early gametogenesis” with an oocyte diameter mean of 8.47±4.6 μm, (2) “Growing stage” with a diameter mean of 21.4±8.4 μm, (3) “Mature stage” with a mean of 36±4.4 μm and (4) “Degenerating stage” with 46±7.3 μm in mean diameter. This scale was generated from a modal distribution analysis (interactive program for fitting mixtures of distributions) and microscopic descriptions of gonad characteristics. The use of this scale for reproductive studies in C. gigas is discussed.