Long-term glial reactivity in rat retinas ipsilateral and contralateral to experimental glaucoma
Long-term glial reactivity in rat retinas ipsilateral and contralateral to experimental glaucoma
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DOI：
10.1016/j.exer.2005.01.012
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Negi, A
中科院分区：
文献类型：
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作者：
Kanamori, A;Nakamura, M;Negi, A
尽管已知青光眼会改变神经胶质细胞的反应性，但眼内压（IOP）升高对神经胶质细胞变化的长期影响尚未完全阐明。本研究旨在探讨单侧眼中巩膜外静脉烧灼 (EVC) 引起的慢性眼压升高如何影响治疗组和对侧眼中星形胶质细胞和 Muller 细胞的反应性。在 6 个月前的几个时间点制作平装视网膜制剂，并对其进行神经胶质原纤维酸性蛋白 (GFAP) 的免疫染色。对视网膜匀浆进行一维或二维电泳，然后进行 GFAP 免疫印迹。EVC 使 IOP 显着增加，从 13.1 mmHg 升至 27.8，并随着时间的推移逐渐下降。在平装视网膜中，烧灼后 3 天，星形胶质细胞丢失，但 Muller 细胞获得 GFAP 免疫反应性。随着时间的推移，神经胶质细胞的变化部分逆转，但即使在眼压正常化后仍持续存在。在对侧眼中，EVC后1个月及此后逐渐出现类似的神经胶质变化。免疫印迹显示，与年龄匹配的对照视网膜相比，治疗组和对侧视网膜中的 GFAP 不仅分子大小发生了变化，而且等电聚焦也发生了改变。EVC 导致星形胶质细胞和 Muller 细胞中 GFAP 免疫反应性发生相反的反应。迟发性神经胶质反应也发生在对侧视网膜中。 (c) 2005 Elsevier Ltd. 保留所有权利。
Although glaucoma is known to alter glial reactivity, the long-term effect of elevated intraocular pressure (IOP) on glial change has not been fully elucidated. This study aimed to examine how chronically elevated IOP induced by episcleral vein cauterization (EVC) in unilateral eyes affect reactivities of astrocytes and Muller cells of rats in the treated as well as contralateral eyes over time.EVC in unilateral eyes of Sprague-Dawley rats were performed to produce chronically elevated IOP. Flat mounted retina preparations were made at several points until 6 months, which were subjected to immunostaining for glial fibrillary acidic protein (GFAP). Retinal homogenates were one- or two-dimensionally electrophoresed, followed by GFAP immunoblotting.EVC significantly increased IOPs up to 27.8 from 13.1 mmHg, which gradually decreased over time. In flat mounted retinas, astrocytes lost but Muller cells gained GFAP immunoreactivity at 3 days after cauterization. The glial changes were partially reversed over time but last even after IOP normalization. In the contralateral eyes, similar glial changes gradually appeared at 1 month after EVC and thereafter. Immunoblotting demonstrated not only molecular size shifts but also alteration of isoelectric focusing of GFAP both in treated and contralateral retina as compared with age-matched control retina.EVC led to opposite reactions in astrocytes and Muller cells in terms of GFAP immunoreactivity. Late-onset glial reactivity also occurred in the contralateral retina. (c) 2005 Elsevier Ltd. All rights reserved.