KERATINOCYTES ARE INVOLVED IN REGULATING THE DEVELOPMENTAL-CHANGES IN THE PROLIFERATIVE ACTIVITY OF MOUSE EPIDERMAL MELANOBLASTS IN SERUM-FREE CULTURE
KERATINOCYTES ARE INVOLVED IN REGULATING THE DEVELOPMENTAL-CHANGES IN THE PROLIFERATIVE ACTIVITY OF MOUSE EPIDERMAL MELANOBLASTS IN SERUM-FREE CULTURE
复制标题
DOI：
10.1006/dbio.1994.1007
复制
发表时间：
1994-01-01
影响因子：
2.7
通讯作者：
HIROBE, T
中科院分区：
文献类型：
--
作者：
HIROBE, T
当将来自0.5、2.5和4.5日龄小鼠的表皮细胞悬液接种在未涂覆的聚苯乙烯培养皿上，并用补充有二丁酰环腺苷3′，5 ′-单磷酸和碱性成纤维细胞生长因子的无血清培养基培养时，成黑素细胞在角质形成细胞集落周围显著增殖，并且在12-14天后，成黑素细胞的纯培养物和富集培养物（约100 μ g/ml）中，75%）和黑素细胞（约。25%）。相反，当来自7.5-，20.5-，和60.5日龄小鼠的表皮细胞悬浮液进行类似的培养时，角质形成细胞未能附着在培养皿上，成黑素细胞根本没有增殖。然而，当将老年小鼠的表皮细胞悬液接种在I型胶原包被的培养皿上并进行类似培养时，角质形成细胞良好地附着在培养皿上，成黑素细胞在角质形成细胞集落周围急剧增殖。此外，纯黑素母细胞和黑素细胞来源于年轻和年老的小鼠的原代培养物可以在胶原蛋白包被的培养皿上与培养基一起在存在从纯的原代角质形成细胞群体传代培养的次级角质形成细胞的情况下传代培养。未观察到年轻和老年小鼠之间的次级成黑素细胞的增殖活性的差异。这些结果表明，角质形成细胞参与调节小鼠表皮成黑素细胞的增殖，并在培养中的表皮成黑素细胞的增殖活性的发育变化是由于角质形成细胞的基板附着和增殖的发育变化，而不是成黑素细胞的内在变化。
When epidermal cell suspensions derived from 0.5-, 2.5-, and 4.5-day-old mice were plated onto uncoated polystyrene dishes and cultured with serum-free medium supplemented with dibutyryl cyclic adenosine 3′,5′-monophosphate and basic fibroblast growth factor, melanoblasts proliferated dramatically around keratinocyte colonies and after 12-14 days pure and enriched cultures of melanoblasts (ca. 75%) and melanocytes (ca. 25%) were obtained. In contrast, when epidermal cell suspensions derived from 7.5-, 20.5-, and 60.5-day-old mice were cultured similarly, keratinocytes failed to attach to the dish and melanoblasts did not proliferate at all. However, when epidermal cell suspensions of older mice were plated onto type I collagen-coated dishes and cultured similarly, keratinocytes attached well to the dish and melanoblasts proliferated dramatically around keratinocyte colonies. Moreover, pure melanoblasts and melanocytes derived from primary cultures of young and old mice could be subcultured on collagen-coated dishes with the medium in the presence of secondary keratinocytes that were subcultured from a pure population of primary keratinocytes. No differences were observed in the proliferative activity of secondary melanoblasts between young and old mice. These results suggest that keratinocytes are involved in regulating the proliferation of mouse epidermal melanoblasts and that the developmental changes in the proliferative activity of epidermal melanoblasts in culture are due to the developmental changes in the substrate attachment and proliferation of keratinocytes, rather than to intrinsic changes in melanoblasts.