Functional and Structural Characterization of P[19] Rotavirus VP8☆ Interaction with Histo-blood Group Antigens
Functional and Structural Characterization of P[19] Rotavirus VP8☆ Interaction with Histo-blood Group Antigens
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P[19]轮状病毒 VP8 与组织血型抗原相互作用的功能和结构特征。
DOI：
10.1128/jvi.01566-16
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发表时间：
2016-11-01
影响因子：
5.4
通讯作者：
Duan, Zhao-Jun
中科院分区：
文献类型：
--
作者：
Sun, Xiaoman;Li, Dandi;Duan, Zhao-Jun
A种轮状病毒（RVs）是急性胃肠炎的主要病原体。最近，组织血型抗原（HBGAs）被报道与人RVA VP8（星）蛋白相互作用。人P[19]是一种罕见的猪源P基因型，偶有感染人类。p[19] VP8（星）与HBGAs相互作用的功能和结构特征尚不清楚。在本研究中，我们表达并纯化了人和猪p[19] rv的VP8（star）蛋白。在寡糖和唾液结合实验中，P[19] VP8（star）蛋白与A型、B型和o型唾液样品明显结合，而与分泌状态无关，这意味着广泛的结合模式。然而，它们没有显示出与任何低聚糖的特异性结合。此外，我们在2.4埃处解算了人类P[19] VP8（星）的结构，显示出典型的半乳糖凝集素样褶皱。结构比对表明，P[19] VP8（星）与P[8]最相似，这与系统发育分析一致。结构叠加揭示了缺乏与低聚糖结合的基础。我们的研究表明，p[19] RVs可能与其他低聚糖或配体结合，并可能在人类中广泛传播。因此，有必要对p[19]rv的流行和演变进行监测。
Rotaviruses (RVs) of species A (RVA) are a major causative agent of acute gastroenteritis. Recently, histo-blood group antigens (HBGAs) have been reported to interact with human RVA VP8(star) proteins. Human P[19] is a rare P genotype of porcine origin that infects humans sporadically. The functional and structural characteristics of P[19] VP8(star) interaction with HBGAs are unknown. In this study, we expressed and purified the VP8(star) proteins of human and porcine P[19] RVs. In oligosaccharide and saliva binding assays, P[19] VP8(star) proteins showed obvious binding to A-, B-, and O-type saliva samples irrespective of the secretor status, implying broad binding patterns. However, they did not display specific binding to any of the oligosaccharides tested. In addition, we solved the structure of human P[19] VP8(star) at 2.4 angstrom, which revealed a typical galectin-like fold. The structural alignment demonstrated that P[19] VP8(star) was most similar to that of P[8], which was consistent with the phylogenetic analysis. Structure superimposition revealed the basis for the lack of binding to the oligosaccharides. Our study indicates that P[19] RVs may bind to other oligosaccharides or ligands and may have the potential to spread widely among humans. Thus, it is necessary to place the prevalence and evolution of P[19] RVs under surveillance.