Rapid O serogroup identification of the six clinically relevant Shiga toxin-producing Escherichia coli by antibody microarray
Rapid O serogroup identification of the six clinically relevant Shiga toxin-producing Escherichia coli by antibody microarray
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DOI：
10.1016/j.mimet.2013.03.024
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发表时间：
2013-06-01
影响因子：
2.2
通讯作者：
DebRoy, Chitrita
中科院分区：
文献类型：
--
作者：
Hegde, Narasimha V.;Praul, Craig;DebRoy, Chitrita
建立了一种抗体微阵列，用于检测美国农业部食品安全和检验局宣布为肉类掺假的产滋贺毒素大肠杆菌（STEC）的“前六位”非0157血清群O26、O 45、O 103、O 111、O 121和O 145。该芯片的灵敏度为10（5）CFU，在人工接种的绞细牛肉中的每个血清群的检测限为1-10 CFU的富集12小时后。评估用于打印和标记的抗体的最佳浓度以及用于结合抗体的细菌稀释液。该阵列使用最少量的抗体和其他试剂，并且可以用于在少于3小时内直接从富集的样品中平行筛选STEC的多个靶O基团。此外，抗体阵列提供了包括其他O血清群的E。大肠杆菌中表达，可用于高通量筛选。该方法适用于大肠杆菌致病性STECO群的检测。我们亦会继续致力改善肉类及其他食物中的大肠杆菌含量，从而改善食物安全及公众健康。(C)2013爱思唯尔有限公司版权所有。
An antibody microarray was developed to detect the "top six" non-0157 serogroups, O26, O45, O103, O111, O121, and O145 of Shiga toxin-producing Escherichia coli (STEC), that have been declared as adulterant in meat by the Food Safety and Inspection Service of the United States Department of Agriculture. The sensitivity of the array was 10(5) CFU and the limit of detection of each serogroup in artificially inoculated ground beef was 1-10 CFU following 12 h of enrichment. Optimal concentrations of antibodies for printing and labeling and bacterial dilutions for binding to the antibodies were assessed. The array utilized a minimal amount of antibodies and other reagents and may be utilized for screening of multiple target O groups of STEC in parallel, directly from enriched samples in less than 3 h. Furthermore, the antibody array provides the flexibility to include other O serogroups of E. coli and may be adopted for high throughput screening. The method is potentially applicable to detect the pathogenic STEC O groups of E. coli in meat and other food, thus improving food safety and public health. (C) 2013 Elsevier B.V. All rights reserved.