Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on TNF-alpha-dependent IL-6 secretion in normal human dermal fibroblasts.
Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on TNF-alpha-dependent IL-6 secretion in normal human dermal fibroblasts.
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发表时间：
2012-11
影响因子：
0.4
通讯作者：
A. Gruchlik;M. Jurzak;E. Chodurek;Z. Dzierżewicz
中科院分区：
文献类型：
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作者：
A. Gruchlik;M. Jurzak;E. Chodurek;Z. Dzierżewicz
药妆品代表了化妆品和药品的结合。有许多药妆活性产品，可大致分为以下类别：抗氧化剂，寡肽，生长因子和色素闪电剂。Gly-His-Lys（GHK）和Gly-Gly-His（GGH）三肽及其铜配合物由于具有较高的活性和良好的皮肤耐受性而备受关注。最近的研究表明，它们在伤口愈合、组织修复和皮肤炎症过程中发挥着重要的生理作用。这些肽的抗炎特性的机制尚不清楚。本研究的目的是评价两种肽GGH的影响。GHK及其铜配合物和酵母菌/铜发酵物（Oligolides Copper）对正常人皮肤成纤维细胞NHDF细胞系中促炎性IL-6分泌的影响使用ELISA试剂盒评估IL-6。GGH、GHK、CuCl 2及其铜络合物降低成纤维细胞中TNF-α依赖性IL-6分泌。IL-6对正常伤口愈合、皮肤炎症和UVB诱导的红斑至关重要。由于铜肽的抗炎特性，可用于皮肤表面，代替副作用较大的皮质类固醇或非甾体抗炎药。我们的观察提供了一些新的信息，这些三肽在皮肤炎症中的作用。
Cosmeceuticals represent a marriage between cosmetics and pharmaceuticals. There are numerous cosmeceutically active products which can be broadly classified into the following categories: antioxidants, oligopeptides, growth factors and pigment lightning agents. Much attention has been focused on the tripeptides such as Gly-His-Lys (GHK) and Gly-Gly-His (GGH) and their copper complexes, which have a high activity and good skin tolerance. Recent data suggested their physiological role in process of wound healing, tissue repair and skin inflammation. The mechanism of anti-inflammatory properties of these peptides is not clear. The aim of the study was evaluation of influence of two peptides GGH. GHK and their copper complexes and saccharomyces/copper ferment (Oligolides Copper) on secretion of pro-inflammatory IL-6 in normal human dermal fibroblasts NHDF cell line. IL-6 was evaluated using the ELISA kit. GGH, GHK, CuCl2 and their copper complexes decreased TNF-alpha-dependent IL-6 secretion in fibroblasts. IL-6 is crucial for normal wound healing, skin inflammation and UVB-induced erythema. Because of the anti-inflammatory properties, the copper-peptides could be used on the skin surface instead of corticosteroids or non-steroidal anti-inflammatory drugs, which have more side effects. Our observations provide some new information about the role of these tripeptides in skin inflammation.