Genome-wide analysis of chromatin status using tiling microarrays.
Genome-wide analysis of chromatin status using tiling microarrays.
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DOI：
10.1016/j.ymeth.2006.11.002
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发表时间：
2007-03
期刊：
影响因子：
4.8
通讯作者：
S. Shivaswamy;V. Iyer
中科院分区：
文献类型：
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作者：
S. Shivaswamy;V. Iyer
真核基因组被包装成染色质，染色质修饰和重塑在转录调控、DNA复制、重组和修复中发挥重要作用。最近的研究结果表明，各种翻译后组蛋白修饰协同招募不同的效应蛋白，从而引起核小体的动员并引起不同的下游后果。使用针对核心组蛋白或特定组蛋白修饰的抗体的染色质免疫沉淀 (ChIP) 与高分辨率平铺微阵列分析相结合，可以检查全基因组范围内的核小体占据和组蛋白修饰状态。将全基因组染色质状态与全局基因表达模式进行比较可以揭示组蛋白修饰的特定模式与由此产生的基因表达之间的因果关系。在这里，我们描述了基于微阵列技术最新进展的当前方法来进行此类研究。
The eukaryotic genome is packaged into chromatin, and chromatin modification and remodeling play an important role in transcriptional regulation, DNA replication, recombination and repair. Recent findings have shown that various post-translational histone modifications cooperate to recruit different effector proteins that bring about mobilization of the nucleosomes and cause distinct downstream consequences. The combination of chromatin immunoprecipitation (ChIP) using antibodies directed against the core histones or specific histone modifications, with high-resolution tiling microarray analysis allows the examination of nucleosome occupancy and histone modification status genome-wide. Comparing genome-wide chromatin status with global gene expression patterns can reveal causal connections between specific patterns of histone modifications and the resulting gene expression. Here, we describe current methods based on recent advances in microarray technology to conduct such studies.