Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration: Characterization and in vitro biological behavior
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration: Characterization and in vitro biological behavior
复制标题
DOI：
10.1016/j.dental.2013.01.009
复制
发表时间：
2013-04-01
期刊：
影响因子：
5
通讯作者：
Mano, Joao F.
中科院分区：
文献类型：
--
作者：
Leal, Ana I.;Caridade, Sofia G.;Mano, Joao F.
Objective.在牙周缺损的治疗中，复合膜可用于保护损伤区域，同时刺激组织再生。本工作描述了具有不对称生物活性的聚（D，L-乳酸）/生物玻璃（R）（PDLLA/BG）复合膜的开发和表征。我们假设BG微粒的存在可以增强纯PDLLA膜的结构和骨传导性能。通过调节的溶剂流延方法制备膜，该方法促进无机组分沿膜厚度沿着的不均匀分布。评价了所开发生物材料的体外生物活性行为（在模拟体液（SBF）中浸泡后磷灰石层的沉淀）、SEM观察、FTIR、溶胀、重量损失和机械性能。用人骨髓基质细胞和人牙周膜细胞评估细胞在膜上的行为。仅复合膜的富含BG的表面诱导类骨磷灰石在SBF中沉淀，表明该生物材料表现出不对称的骨传导性质。SEM图像、DNA含量和代谢活性定量显示复合膜上的细胞粘附和增殖得到改善。复合膜还刺激细胞分化、矿化以及细胞外基质和钙结节的产生，表明在PDLLA基质中加入生物活性微粒的积极作用。结果表明，所提出的非对称PDLLA/BG膜可能有潜力用于引导组织再生治疗或骨科应用，并改善结局。(C)2013年牙科材料学院。由爱思唯尔有限公司出版。保留所有权利。
Objective. In the treatment of periodontal defects, composite membranes might be applied to protect the injured area and simultaneously stimulate tissue regeneration. This work describes the development and characterization of poly(D,L-lactic acid)/Bioglass (R) (PDLLA/BG) composite membranes with asymmetric bioactivity. We hypothesized that the presence of BG microparticles could enhance structural and osteoconductivity performance of pure PDLLA membranes.Methods. The membranes were prepared by an adjusted solvent casting method that promoted a non-uniform distribution of the inorganic component along the membrane thickness. In vitro bioactive behavior (precipitation of an apatite layer upon immersion in simulated body fluid, SBF), SEM observation, FTIR, swelling, weight loss and mechanical properties of the developed biomaterials were evaluated. Cell behavior on the membranes was assessed using both human bone marrow stromal cells and human periodontal ligament cells.Results. Just the BG rich face of the composite membranes induced the precipitation of bone-like apatite in SBF, indicating that this biomaterial exhibit asymmetric osteoconductive properties. SEM images, DNA content and metabolic activity quantification revealed an improved cell adhesion and proliferation on the composite membranes. Composite membranes also stimulated cell differentiation, mineralization, and production of extracellular matrix and calcium nodules, suggesting the positive effect of adding the bioactive microparticles in the PDLLA matrix.Significance. The results indicate that the proposed asymmetric PDLLA/BG membranes could have potential to be used in guided tissue regeneration therapies or in orthopedic applications, with improved outcomes. (C) 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.