Association mapping for pre-harvest sprouting resistance in white winter wheat
Association mapping for pre-harvest sprouting resistance in white winter wheat
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DOI：
10.1007/s00122-012-1872-0
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发表时间：
2012-08-01
影响因子：
5.4
通讯作者：
Sorrells, Mark E.
中科院分区：
文献类型：
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作者：
Kulwal, Pawan;Ishikawa, Goro;Sorrells, Mark E.
关联图谱鉴定了198个基因型的白冬小麦收获前发芽抗性的数量性状位点（qtl）和相关标记。共有1166个标记位点，包括DArT和SSR标记，代表小麦全部21条染色体。采用一般线性模型和混合线性模型对4年来收集的小灵通数据进行分析。关联分析鉴定出与7条染色体上13个标记相关的8个qtl。在2B染色体臂各检测到1个QTL，在1BS、2DS、4AL、6DL、7BS和7DS染色体臂各检测到1个QTL。除了7BS上的QTL外，所有QTL都位于与先前报告相似的位置，如果得到证实，7BS上的QTL很可能是新的。主成分和亲属矩阵被用来解释种群结构的存在，但对结果的影响很小。虽然没有一个QTL在所有环境中都是高度显著的，但在三种不同的环境中检测到4A染色体长臂上的QTL，并且也使用了多年来最好的线性无偏预测。虽然以前的报告已经确定这是一个主要的QTL，但在我们的双亲本群体中，它的影响很小。本研究的结果强调了关联图谱的好处，以及在植物育种计划中使用精英材料的关联图谱的价值。
Association mapping identified quantitative trait loci (QTLs) and the markers linked to pre-harvest sprouting (PHS) resistance in an elite association mapping panel of white winter wheat comprising 198 genotypes. A total of 1,166 marker loci including DArT and SSR markers representing all 21 chromosomes of wheat were used in the analysis. General and mixed linear models were used to analyze PHS data collected over 4 years. Association analysis identified eight QTLs linked with 13 markers mapped on seven chromosomes. A QTL was detected on each arm of chromosome 2B and one each on chromosome arms 1BS, 2DS, 4AL, 6DL, 7BS and 7DS. All except the QTL on 7BS are located in a location similar to previous reports and, if verified, the QTL on 7BS is likely to be novel. Principal components and the kinship matrix were used to account for the presence of population structure but had only a minor effect on the results. Although, none of the QTLs was highly significant across all environments, a QTL on the long arm of chromosome 4A was detected in three different environments and also using the best linear unbiased predictions over years. Although previous reports have identified this as a major QTL, its effects were minor in our biparental mapping populations. The results of this study highlight the benefits of association mapping and the value of using elite material in association mapping for plant breeding programs.