Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter
Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter
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DOI：
10.1021/pr060624p
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Klug, Gabriele
中科院分区：
文献类型：
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作者：
Glaeser, Jens;Zobawa, Monica;Klug, Gabriele
单线态氧（O-1（2））是兼性光合细菌类球红细菌（Rhodobacter sphaeroides）中的一种胁迫因子和信号。用L-[S-35]-甲硫氨酸进行的体内蛋白质标记和二维凝胶电泳分析显示，61种蛋白质的合成响应O-1而发生变化（2）。O-1（2）处理后，蛋白质合成模式与H_2O_2处理后不同，但与强光照射后相似。这表明对不同活性氧（ROS）具有选择性的调节机制和对光的响应部分由O-1介导（2）。对突变菌株的分析支持对O-1（2）的反应主要受rpoE（sigma（E））调节，但也受其他因素对sigma（E）依赖性反应的调节，并存在sigma（E）独立性反应。RNA伴侣分子Hfq参与O-1（2）反应，暗示了小调控RNA的作用。
Singlet oxygen (O-1(2)) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with L-[S-35]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to O-1(2). After O-1(2) treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by O-1(2). Analysis of mutant strains support that the response to O-1(2) is regulated mainly by rpoE (sigma(E)), but also a modulation of the sigma(E) dependent response by other factors and the existence of sigma(E) independent responses. The involvement of the RNA chaperon Hfq in the O-1(2) response implies a role of small regulatory RNAs.