Differential expression of cannabinoid CB2 receptor mRNA in mouse immune cell subpopulations and following B cell stimulation
Differential expression of cannabinoid CB2 receptor mRNA in mouse immune cell subpopulations and following B cell stimulation
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DOI：
10.1016/s0014-2999(01)01122-0
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发表时间：
2001-07-06
影响因子：
5
通讯作者：
Klein, TW
中科院分区：
文献类型：
--
作者：
Lee, SF;Newton, C;Klein, TW
据报道，大麻素CB 2受体在不同的人免疫亚群中以不同的量表达。为了检测CB的表达模式，在小鼠中，免疫细胞亚群被纯化并通过半定量逆转录-聚合酶链反应（RT-PCR）进行研究。CB 2 mRNA在脾B细胞中含量最高，其次是巨噬细胞和T细胞。此外，CB 2在巯基乙酸盐诱导的腹腔巨噬细胞中表达，但在驻留的腹腔巨噬细胞中不表达。除了对基础活性下受体表达的这些研究外，还研究了免疫细胞激活后CBI mRNA的表达。细菌脂多糖刺激以剂量-反应方式下调脾细胞培养物中CB 2 mRNA的表达，而使用抗CD 40抗体通过分化簇40（CD 40）刺激上调反应，与白细胞介素-4共刺激减弱抗CD 40反应。这些结果表明，CB 2 mRNA表达在小鼠免疫亚群中存在差异，与在人免疫细胞中观察到的相似。此外，结果表明，脂多糖和抗CD 40激活的信号通路可能对CB 2 mRNA表达产生不同的影响。(C)出版社：Elsevier Science B. V.
Cannabinoid CB2 receptor is reported to be expressed in varying amounts in different human immune subpopulations. To examine the expression pattern of CB, in the mouse, immune cell subpopulations were purified and studied by semiquantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR). CB2 mRNA was most abundant in splenic B cells, followed by macrophages and T cells. Furthermore, CB2 was expressed in thioglycollate-elicited peritoneal macrophages, but not in resident peritoneal macrophages. In addition to these studies on receptor expression at basal activity, CBI mRNA expression was also studied following immune cell activation. Bacterial lipopolysaccharide stimulation downregulated CB2 mRNA expression in splenocyte cultures in a dose-response manner, while stimulation through cluster of differentiation 40 (CD40) using anti-CD40 antibody upregulated the response and costimulation with interleukin-4 attenuated the anti-CD40 response. These results demonstrate that CB2 mRNA expression differs among mouse immune subpopulations similar to what is observed in human immune cells. Furthermore, the results suggest that the signaling pathways activated by lipopolysaccharide and anti-CD40 might have different effects on CB2 mRNA expression. (C) 2001 Published by Elsevier Science B.V.