GENE-20 PRODUCT OF BACTERIOPHAGE-T4 ITS PURIFICATION AND STRUCTURE
GENE-20 PRODUCT OF BACTERIOPHAGE-T4 ITS PURIFICATION AND STRUCTURE
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DOI：
10.1016/0022-2836(81)90121-2
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
VANDRIEL, R
中科院分区：
文献类型：
--
作者：
DRIEDONKS, RA;ENGEL, A;VANDRIEL, R
描述了基因20产物的纯化方法，所述基因20产物是噬菌体T4头部的定量次要但必需的蛋白质。在纯化过程中并响应不同的溶剂条件，蛋白质表现得就像是疏水性的。其结构进行了研究，通过常规和扫描透射电子显微镜和沉降分析。基因20产物以十二聚体结构存在，具有12重旋转对称性和约MW。740，000，通过2种独立的方法（电子散射和沉降平衡）测量。它由14 nm长和7 nm直径的中空圆柱体和12个径向突起组成，每个突起的尺寸为λ。7nm ×5nm。观察到双环和环的微晶。微晶的尺寸为. apprx。1 μ m，并且具有体心四面体型晶格。基因20产物寡聚体的结构，特别是其对称性，对T4头部的组装具有影响，其壳具有5重对称性。
A purification method is described for gene 20 product which is a quantitatively minor, but essential, protein of the bacteriophage T4 head. During purification and in response to different solvent conditions the protein behaves as if it were hydrophobic. Its structure was studied by conventional and scanning transmission electron microscopy and sedimentation analysis. Gene 20 product exists as a dodecameric structure with 12-fold rotational symmetry and a MW of .apprx. 740,000 as measured by 2 independent methods (electron scattering and sedimentation equilibrium). It is composed of a hollow cylinder, 14 nm long and 7 nm diameter, and 12 radial protrusions, each measuring .apprx. 7 nm .times. 5 nm. Double rings and microcrystals of rings were observed. The microcrystals measure .apprx. 1 .mu.m and have a body-centered tetragonal type of lattice. The structure of the gene 20 product oligomer, in particular its symmetry, has consequences for the assembly of the T4 head, whose shell has 5-fold symmetry.