Rupture force measurement of biotin-streptavidin bonds using optical trapping
Rupture force measurement of biotin-streptavidin bonds using optical trapping
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DOI：
10.1063/1.1999855
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发表时间：
2005-07-25
影响因子：
4
通讯作者：
Kawata, S
中科院分区：
文献类型：
--
作者：
Ota, T;Sugiura, T;Kawata, S
光学捕获已被应用于研究生物素连接到微粒和链霉亲和素连接到基板之间的键断裂力。通过从基底上拉动颗粒来垂直加载用于破坏键的力，并通过测量颗粒从光阱中的平衡位置的位移来检测。位移通过检测光来测量，所述光是在渐逝场中从颗粒散射的光，并且所述场是通过从高NA物镜透镜输出的光束的全内反射产生的。从破裂力测量产生的直方图中，最常见的破裂力被确定为在3.6和5.4 pN之间，加载速率为7.7 pN/s。(c)2005年美国物理学会。
Optical trapping has been applied to investigate the bond rupture force between biotin attached to a microparticle and streptavidin attached to a substrate. The force for rupturing the bonds was loaded vertically by pulling the particle from the substrate and detected by measuring the displacement of the particle from the equilibrium position in the optical trap. The displacement was measured by detecting light the light scattered from the particle in an evanescent field, and the field was generated by total internal reflection of a beam output from a high-NA objective lens. From the histogram yielded from the rupture force measurements, the most frequent rupture force was determined to be between 3.6 and 5.4 pN with a loading rate of 7.7 pN/s. (c) 2005 American Institute of Physics.