3′-Deoxy-3′-[18F]-fluorothymidine ([18F]-FLT) transport in newly diagnosed glioma: correlation with nucleoside transporter expression, vascularization, and blood-brain barrier permeability
3′-Deoxy-3′-[18F]-fluorothymidine ([18F]-FLT) transport in newly diagnosed glioma: correlation with nucleoside transporter expression, vascularization, and blood-brain barrier permeability
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DOI：
10.1007/s10014-013-0136-2
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发表时间：
2013-10-01
影响因子：
3.3
通讯作者：
Tamiya, Takashi
中科院分区：
文献类型：
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作者：
Shinomiya, Aya;Miyake, Keisuke;Tamiya, Takashi
3'-Deoxy-3'-[F-18]-氟胸苷 ([F-18]-FLT) 是细胞增殖的标志物，已用于神经胶质瘤的正电子发射断层扫描 (PET) 检查。本研究的目的是探讨神经胶质瘤中 [F-18]-FLT 的摄取是否与平衡核苷转运蛋白 1 (ENT1) 的信使 RNA (mRNA) 水平、微血管密度（通过 CD34 免疫组织化学评估）和血脑屏障 (BBB) 破坏相关。共有 21 名新诊断的神经胶质瘤患者接受了 [F-18]-FLT PET 检查。肿瘤病变被确定为 [F-18]-FLT 摄取局部增加的区域，超过周围正常组织。 [F-18]-FLT PET 的动态分析揭示了磷酸化速率常数 k (3) 与 ENT1 表达之间的相关性；然而，动力学参数和 CD34 评分之间没有相关性。钆 (Gd) 增强评分（评估 BBB 破裂）与 ENT1 表达、CD34 评分和 Ki-67 指数之间存在良好的相关性。这项初步研究表明，由于神经胶质瘤中 BBB 通透性，ENT1 表达可能无法反映体内 [F-18]-FLT 的积累。
3'-Deoxy-3'-[F-18]-fluorothymidine ([F-18]-FLT), a marker of cellular proliferation, has been used in positron emission tomography (PET) examination of gliomas. The aim of this study was to investigate whether the uptake of [F-18]-FLT in glioma correlates with messenger RNA (mRNA) levels of the equilibrative nucleoside transporter 1 (ENT1), microvascular density (assessed by CD34 immunohistochemistry), and the blood-brain barrier (BBB) breakdown. A total of 21 patients with newly diagnosed glioma were examined with [F-18]-FLT PET. Tumor lesions were identified as areas of focally increased [F-18]-FLT uptake, exceeding that of surrounding normal tissue. Dynamic analysis of [F-18]-FLT PET revealed correlations between the phosphorylation rate constant k (3) and ENT1 expression; however there was no correlation between the kinetic parameters and CD34 score. There was a good correlation between the gadolinium (Gd) enhancement score (evaluating BBB breakdown) and ENT1 expression, CD34 score, and Ki-67 index. This preliminary study suggests that ENT1 expression might not reflect accumulation of [F-18]-FLT in vivo due to BBB permeability in glioma.