Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection
Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection
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DOI：
10.1038/s41598-019-42919-y
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发表时间：
2019-05-01
影响因子：
4.6
通讯作者：
Heaney, Christopher D.
中科院分区：
文献类型：
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作者：
Randad, Pranay R.;Dillen, Carly A.;Heaney, Christopher D.
工业生猪操作（IHO）工人鼻腔携带金黄色葡萄球菌的风险增加，特别是家畜相关（LA）和多重耐药菌株。LA-S的致病性。目前尚不清楚金黄色葡萄球菌的菌株，一些先前的研究表明，与社区相关的耐甲氧西林（CA-MRSA）金黄色葡萄球菌相比，人类的传播和毒力更低。本研究的目的是确定LA-S。在小鼠皮肤和软组织感染（SSTI）模型中，IHO工人感染的金黄色葡萄球菌菌株相对于具有代表性的CA-MRSA菌株引起疾病。感染CC398 LA-S的小鼠。金黄色葡萄球菌菌株（IHW398-1和IHW398-2）比CA-MRSA （SF8300）感染的小鼠病变面积更大，细菌负担更高（p < 0.05）。在一名IHO工作人员中，CC398菌株产生了复发性SSTI，病变大小和细菌负担最大。LA-S。与ca - mrsa感染小鼠相比，金黄色葡萄球菌感染小鼠IL-1 β蛋白水平降低（p < 0.05），表明宿主对LA-S的反应不理想。球菌SSTIs。WGSA显示LA-S携带的毒力因子和耐药基因具有异质性。金黄色葡萄球菌和CA-MRSA菌株观察到的致病性表明，应更加重视防止LA-S的传播。金黄色葡萄球菌进入人类种群。
Industrial hog operation (IHO) workers are at increased risk of carrying Staphylococcus aureus in their nares, particularly strains that are livestock-associated (LA) and multidrug-resistant. The pathogenicity of LA-S. aureus strains remains unclear, with some prior studies suggesting reduced transmission and virulence in humans compared to community-associated methicillin-resistant (CA-MRSA) S. aureus. The objective of this study was to determine the degree to which LA-S. aureus strains contracted by IHO workers cause disease relative to a representative CA-MRSA strain in a mouse model of skin and soft tissue infection (SSTI). Mice infected with CC398 LA-S. aureus strains (IHW398-1 and IHW398-2) developed larger lesion sizes with higher bacterial burden than mice infected with CA-MRSA (SF8300) (p < 0.05). The greatest lesion size and bacterial burden was seen with a CC398 strain that produced a recurrent SSTI in an IHO worker. The LA-S. aureus infected mice had decreased IL-1 beta protein levels compared with CA-MRSA-infected mice (p < 0.05), suggesting a suboptimal host response to LA-S. aureus SSTIs. WGSA revealed heterogeneity in virulence factor and antimicrobial resistance genes carried by LA-S. aureus and CA-MRSA strains. The observed pathogenicity suggest that more attention should be placed on preventing the spread of LA-S. aureus into human populations.