Transcriptomic and hormone analyses reveal mechanisms underlying petal elongation in Chrysanthemum morifolium 'Jinba'
Transcriptomic and hormone analyses reveal mechanisms underlying petal elongation in Chrysanthemum morifolium 'Jinba'
复制标题
转录组和激素分析揭示了菊花‘金巴’花瓣伸长的机制
DOI：
10.1007/s11103-017-0584-x
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发表时间：
2017
影响因子：
5.1
通讯作者：
Chen FD
中科院分区：
文献类型：
--
作者：
Wang Jingjing;Wang Haibin;Ding Lian;Song Aiping;Shen Feng;Jiang Jiafu;Chen Sumei;Chen Fadi;Chen Fadi;Chen FD;Chen FD
关键信息Ausin通过促进细胞伸长来调节菊花花瓣的伸长。转录分析表明，生长素信号转导可能通过TCPs与其他转录因子连接，调节菊花花瓣的伸长。花瓣大小是影响菊花观赏价值的主要因素，但花瓣大小是菊花花瓣发育的机制。桑叶花瓣仍不清楚。在我们的研究中，我们跟踪了花瓣的生长，发现‘金巴’花瓣的基面区域表现出较高的伸长率，在花瓣生长过程中表现出快速的细胞伸长。在花瓣伸长生长过程中，生长素促进了花瓣基底部细胞的伸长和细胞数量的增加。为了进一步研究花瓣生长的分子机制，采用了RNA-seq(高通量cDNA测序)技术。以‘金巴’花发育的现蕾期、断蕾期、初花期和盛花期的花瓣为材料，构建了4个cDNA文库。对差异表达基因的分析表明，生长素是控制花瓣生长的最重要的调节因子。TEOSINTEBRANCHED 1、CycloidEA和PCF转录因子基因(TCPs)、碱性螺旋-环-螺旋编码基因(BHLH)、谷氧还蛋白-C(GRXC)等锌指蛋白基因表达明显上调，可能对‘金巴’花瓣的生长有显著影响。考虑到拟南芥中这些基因之间的相互作用，我们推测生长素信号转导可能通过TCPs与转录因子显示出密切的关系。总而言之，我们提出了第一个全面的转录和激素分析的FC。桑叶花瓣。研究结果为进一步明确菊花花瓣在伸长期的发育机制提供了方向，对改善菊花花瓣的观赏特性具有重要意义。桑叶霉菌的分子育种。
Key messageAuxin regulates chrysanthemum petal elongation by promoting cell elongation. Transcriptomic analysis shows that auxin signal transduction may connect with other transcription factors by TCPs to regulate chrysanthemum petal elongation.AbstractAs an ornamental species,Chrysanthemum morifoliumhas high ornamental and economic value. Petal size is the primary factor that influences the ornamental value of chrysanthemum, but the mechanism underlying the development ofC. morifoliumpetals remains unclear. In our study, we tracked the growth of petals and found that the basal region of ‘Jinba’ petals showed a higher elongation rate, exhibiting rapid cell elongation during petal growth. During petal elongation growth, auxin was demonstrated to promote cell elongation and an increase in cell numbers in the petal basal region. To further study the molecular mechanisms underlying petal growth, the RNA-seq (high-throughput cDNA sequencing) technique was employed. Four cDNA libraries were assembled from petals in the budding, bud breaking, early blooming and full blooming stages of ‘Jinba’ flower development. Analysis of differentially expressed genes (DEGs) showed that auxin was the most important regulator in controlling petal growth. The TEOSINTEBRANCHED 1, CYCLOIDEA and PCF transcription factor genes (TCPs),basic helix-loop-helix-encoding gene (bHLH),glutaredoxin-C(GRXC) and other zinc finger protein genes exhibited obvious up-regulation and might have significant effects on the growth of ‘Jinba’ petals. Given the interaction between these genes inArabidopsis thaliana, we speculated that auxin signal transduction might exhibit a close relationship with transcription factors through TCPs. In summary, we present the first comprehensive transcriptomic and hormone analyses ofC. morifoliumpetals. The results offer direction in identifying the mechanism underlying the development of chrysanthemum petals in the elongated phase and have great significance in improving the ornamental characteristics ofC. morifoliumvia molecular breeding.