Identification of marker proteins by orthodontic treatment: relationship of RANKL in the gingival crevicular fluid and of amylase in whole saliva with orthodontic treatment
Identification of marker proteins by orthodontic treatment: relationship of RANKL in the gingival crevicular fluid and of amylase in whole saliva with orthodontic treatment
复制标题
正畸治疗标记蛋白的鉴定：龈沟液中的RANKL和全唾液中的淀粉酶与正畸治疗的关系
DOI：
10.1007/s10266-013-0121-6
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发表时间：
2014
期刊：
影响因子：
2.5
通讯作者：
Shimomura H.
中科院分区：
文献类型：
--
作者：
Kuroki H;Miyagawa Y;Shimomura-Kuroki J;Endo T;Shimomura H.
进行正畸医疗以将牙齿移动到最佳位置以获得最佳咬合。正畸治疗伴随着由于正畸力引起的机械应力和作为疼痛或不愉快经历的心理应力。本研究的目的是确定在正畸治疗过程中的应力标记蛋白。分析龈沟液（GCF）中NFκB受体激活因子（RANKL）和热休克蛋白70（HSP 70）的水平作为机械应激的标志物，分析全唾液中嗜铬粒蛋白A（CgA）和淀粉酶的水平作为心理应激的标志物。在治疗开始时和治疗后24小时从对照和实验牙齿收集GCF。在治疗前、治疗开始时和治疗后24小时收集全唾液。RANKL在实验组GCF中于治疗后24 h表达，而在对照组GCF中不表达。HSP70在GCF中呈组成性表达，从治疗开始到治疗后24 h，其水平在对照组和实验组之间无明显变化。与对照组相比，治疗后24 h，全唾液中的淀粉酶活性增强，但CgA水平在组间变化不大。这些结果表明，RANKL和淀粉酶可能是候选标记物的机械和心理压力，分别在正畸治疗期间，即使总蛋白浓度和淀粉酶活性显示了一个大的标准差之间的主题。因此，需要进一步的研究来建立这些标记物用于临床。
Orthodontic medical treatment is performed to move a tooth to the optimal position to obtain optimal occlusion. Orthodontic treatment is accompanied by mechanical stress due to orthodontic force and by psychological stress that is experienced as pain or displeasure. The purpose of this study was to identify stress marker proteins during orthodontic treatment. Levels of receptor activator of NFκB (RANKL) and heat shock protein 70 (HSP70) in the gingival crevicular fluid (GCF) were analyzed as markers of mechanical stress, and levels of chromogranin A (CgA) and amylase in whole saliva were analyzed as markers of psychological stress. GCF was collected from control and experimental teeth at initiation of treatment and 24 h after treatment. Whole saliva was collected before treatment, at initiation of treatment and 24 h after treatment. RANKL was expressed at 24 h after treatment in the experimental GCF, but not in the control GCF. HSP70 appeared to be constitutively expressed in GCF, and its levels showed no major change between the control and experimental groups from initiation of treatment to 24 h after treatment. Amylase activity in whole saliva was enhanced at 24 h after treatment compared to control, but CgA levels showed little change between the groups. These results indicated that RANKL and amylase may be the candidate markers for mechanical and psychological stress, respectively, during orthodontic treatment, even though the total protein concentration and amylase activity displayed a large standard deviation among subjects. Further studies are therefore required to establish these markers for clinical use.