Potato cultivar type affects the structure of ammonia oxidizer communities in field soil under potato beyond the rhizosphere
Potato cultivar type affects the structure of ammonia oxidizer communities in field soil under potato beyond the rhizosphere
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DOI：
10.1016/j.soilbio.2012.03.006
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发表时间：
2012-07-01
影响因子：
9.7
通讯作者：
van Elsas, Jan Dirk
中科院分区：
文献类型：
--
作者：
Dias, Armando Cavalcante Franco;Hoogwout, Eelco Frans;van Elsas, Jan Dirk
植物对参与土壤氨氧化的微生物群的影响一直存在争议。以马铃薯为研究对象，研究了不同种植条件下细菌和古细菌氨氧化剂（AOB和AOA）的丰度和群落结构的动态变化。在四倍重复的实验装置中，研究人员使用了六种不同的马铃薯品种，其中包括一种转基因马铃薯。在块状和根际土壤提取微生物群落DNA的基础上，进行AOB、AOA定量PCR和PCR- dgge分析。此外，还利用样品制作和分析了基于amoA基因片段的克隆文库。无论样品类型（块状土壤还是根际土壤）和不同土壤，AOA(10(4) ~ 10（8)个基因拷贝g（-1）干土）的种群大小普遍高于相同样品(约10(4)~ 10（5)个g（-1）干土）的种群大小，导致对数变换比值为> 1.0。在两种土壤中，根际土壤的AOB数量总体上高于块状土壤，而AOA数量则相反。此外，品种类型对两种土壤中AOB和AOA群落结构均有显著影响，且影响范围延伸至根际以外。这种影响在整个生长季节都存在。土壤类型对AOA群落结构影响不显著，但对AOB群落结构影响较小。对AOB群落结构的分析表明，AOB亚群2、3a、3b和4在一个土壤中普遍存在，2和4在另一个土壤中普遍存在。就AOA而言，土壤/沉积物团簇（SS） I、II、III和IV占主导地位。(C) 2012 Elsevier Ltd.版权所有。
The effects of plants on the microbiota involved in the oxidation of ammonia in soils have been controversial. Here, we investigated the dynamics in the abundances and community structures of the bacterial and archaeal ammonia oxidizers (AOB and AOA, respectively) in two fields that were cropped with potato. Six different potato cultivars were used, including a genetically-modified one, in a fourfold replicated experimental set-up. On the basis of bulk and rhizosphere soil extracted microbial community DNA, AOB and AOA quantitative PCR as well as PCR-DGGE were performed. In addition, samples were used for the production and analysis of amoA gene fragment based clone libraries. Regardless of sample type (bulk versus rhizosphere soil) and across soils, the population sizes of AOA (of the order 10(4)-10(8) amoA gene copies g(-1) dry soil), were generally higher than those of AOB in the same samples (about 10(4)-10(5) g(-1) dry soil), resulting in ratio's of log-transformed values > 1.0. Whereas the AOB numbers were generally raised in the rhizosphere versus bulk soils in both soils, the opposite was true for the AOA numbers. Moreover, significant effects of cultivar type on both the AOB and AOA community structures were found in both soils, and these extended to beyond the rhizospheres. The effects were found across the whole growth season. Soil type did not significantly affect the community structures of AOA, but had a small effect on the community structure of AOB. Analysis of the structures of the AOB communities revealed a prevalence of AOB subgroups 2, 3a, 3b and 4 in one field soil and of 2 and 4 in the other one. With respect to the AOA, soil/sediment clusters (SS) I, II, III and IV were found to prevail. (C) 2012 Elsevier Ltd. All rights reserved.