Thalassotalea algicola sp. nov., an alginate-utilizing bacterium isolated from a red alga
Thalassotalea algicola sp. nov., an alginate-utilizing bacterium isolated from a red alga
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海藻藻 sp.
DOI：
10.1007/s10482-021-01562-2
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发表时间：
2021-03-27
影响因子：
2.6
通讯作者：
Du, Zong-Jun
中科院分区：
文献类型：
--
作者：
Lian, Feng-Bai;Jiang, Shan;Du, Zong-Jun
从中国威海近海红藻(紫菜)中分离到一株兼性厌氧细菌M1531(T)。新品系的细胞革兰氏阴性，杆状，单极鞭毛可动，大小约0.6-0.8×2.0-3.0微米。最适生长温度为30℃，含2%(w/v)的氯化钠，最适pH为6.5-7.0。根据16SRRNA基因序列的系统发育分析结果，菌株M1531(T)与真海链霉菌KCTC 42743(T)的亲缘关系较近(96.9%)。基因组测序显示，该菌株的基因组大小为4,061,950个碱基，G+C含量为39.1mol%，以及4个与藻酸盐降解有关的蛋白质编码基因。根据获得的数据，菌株M1531(T)与近缘模式种的ANI值在95-96%以下，dDDH值在23.8%以下。菌株M1531(T)以Q-8为主要异戊二烯对苯二酚，主要脂肪酸为特征3(C-16：1 omega 7c/C-16：1 omega 6c)、C-16：0和特征8(C-18：1 omega 7c/C-18：1 omega 6c)。菌株M1531(T)的极性脂类经鉴定为磷脂酰甘油、磷脂酰乙醇胺、1种未知磷脂、1种未知氨基脂和4种未知脂。根据表型、化学分类、系统发育和基因组分析的结果，菌株M1531(T)为海藻属的一个新种，命名为Thalassotalea algicola sp.11月是提议的。模式菌株为M1531(T)(=MCCC1H00400(T)=KCTC 72865(T))。
A facultatively anaerobic bacterium, strain M1531(T), was isolated from a red alga (Porphyra) at coastal water in Weihai, China. Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 x 2.0-3.0 mu m in size. Optimum growth occurred at 30 degrees C, with 2% (w/v) NaCl and at pH 6.5-7.0. On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531(T) had close relative with Thalassotalea euphylliae KCTC 42743(T) (96.9%). Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate. According to the data obtained, strain M1531(T) shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species. Strain M1531(T) had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C-16:1 omega 7c/C-16:1 omega 6c), C-16:0 and Summed Features 8 (C-18:1 omega 7c/C-18:1 omega 6c) as the major fatty acids. The polar lipids of strain M1531(T) were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids. According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531(T) represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp. nov. is proposed. The type strain is M1531(T) (= MCCC 1H00400(T) = KCTC 72865(T)).