Histochemistry and storage of organic compounds during basidiosporogenesis in the ectomycorrhizal fungus Pisolithus microcarpus
Histochemistry and storage of organic compounds during basidiosporogenesis in the ectomycorrhizal fungus Pisolithus microcarpus
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DOI：
10.1007/s11274-010-0353-3
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发表时间：
2010-10-01
影响因子：
4.1
通讯作者：
Costa, Mauricio Dutra
中科院分区：
文献类型：
--
作者：
da Rocha Campos, Andre Narvaes;Costa, Mauricio Dutra
了解不同有机化合物在小果披碱草担孢子发生过程中的分布和储存情况，对于更好地理解担孢子萌发的过程至关重要。本研究的目的是检测在小果披碱草担孢子发生过程中酚类物质、还原糖、淀粉、糖原、总多糖、RNA和蛋白质的存在和分布。淀粉和还原糖中未检测到真菌担子果，而其他多糖占主导地位的担子果含有未巩固的periidioles的基础上的细胞外基质。在该区域也检测到酚类物质。糖原存在于菌丝、担子和担孢子中，是真菌担子中重要的贮藏物质。在成熟的担孢子中，RNA积累发生在细胞质外围的离散位置，而多糖和蛋白质被证明占主导地位的细胞壁。担孢子内糖原、RNA和蛋白质的存在强烈地表明为将来的萌发提供了条件，并表明其他因素也可能影响担孢子的萌发。
Knowledge on the distribution and storage of different organic compounds during basidiosporogenesis in P. microcarpus is paramount to a better understanding of basidiospore recalcitrance to germination. The objective of this work was to detect the presence and distribution of phenolics, reducing sugars, starch, glycogen, total polysaccharides, RNA, and proteins during P. microcarpus basidiosporogenesis. Starch and reducing sugars were not detected in the fungal basidiocarps, while other polysaccharides predominated in the extracellular matrix at the base of the basidiocarp containing unconsolidated peridioles. Phenolics were also detected in this region. Glycogen was present inside the hyphae, basidia, and basidiospores and constitutes an important storage compound in the fungal basidiocarps. In mature basidiospores, RNA accumulation occurred at discrete locations in the cytoplasmatic periphery, while polysaccharides and proteins were shown to predominate in the cell wall. The presence of glycogen, RNA, and proteins inside the basidiospores strongly indicates provision for future germination and suggests that other factors may also influence basidiospore recalcitrance.