The effect of nicotinamide on unscheduled DNA synthesis in cultured hepatocytes.
The effect of nicotinamide on unscheduled DNA synthesis in cultured hepatocytes.
复制标题
烟酰胺对培养肝细胞中非计划 DNA 合成的影响。
DOI：
10.1016/0006-291x(80)91581-8
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
Pitot,HC
中科院分区：
文献类型：
--
作者：
Althaus,FR;Lawrence,SD;Sattler,GL;Pitot,HC
培养的肝细胞在直接作用的致癌物，甲基甲磺酸酯，和前致癌物，2-乙酰氨基芴，非程序性DNA合成显着增加，当这些细胞培养在培养基中含有25 mM烟酰胺。烟酰胺的这种作用显然与维持细胞内烟酰胺辅酶水平无关。烟酰胺处理的肝细胞中2-乙酰氨基芴介导的非程序DNA合成的增加可以部分归因于维持更高的微粒体代谢该原致癌物的能力。成年大鼠肝细胞通过增加非程序性DNA合成对烟酰胺产生反应的能力在接种后几小时内或从培养基中短暂停用该维生素后丧失。
Unscheduled DNA synthesis of cultured hepatocytes in response to the direct acting carcinogen, methyl methanesulfonate, and to the procarcinogen, 2-acetylaminofluorene, was markedly increased when these cells were cultured in medium containing 25 mM nicotinamide. This effect of nicotinamide was apparently unrelated to the maintenance of intracellular nicotinamide coenzyme levels. The increase in unscheduled DNA synthesis mediated by 2-acetylaminofluorene in nicotinamide-treated hepatocytes could be partially accounted for by the maintenance of higher microsomal capacity for the metabolism of this procarcinogen. The ability of adult rat hepatocytes to respond to nicotinamide by increased unscheduled DNA synthesis was lost within a few hours after plating or upon short withdrawal of this vitamin from the culture medium.