Glypican-3 expression in gastrointestinal and pancreatic epithelial neoplasms
Glypican-3 expression in gastrointestinal and pancreatic epithelial neoplasms
复制标题
DOI：
10.1016/j.humpath.2012.06.016
复制
发表时间：
2013-04-01
期刊：
影响因子：
3.3
通讯作者：
Wu, Tsung-Teh
中科院分区：
文献类型：
--
作者：
Mounajjed, Taofic;Zhang, Lizhi;Wu, Tsung-Teh
磷脂酰肌醇蛋白聚糖-3（GPC 3）是一种质膜结合蛋白聚糖，可在某些恶性肿瘤中过表达，但与肝细胞癌（HCC）特别相关。GPC 3目前被用作HCC的免疫组化标记物，但其在胃肠道（GI）和胰腺（肝转移的常见来源）上皮肿瘤中的表达尚未详细研究。在这项研究中，我们研究了GPC 3免疫反应性98肿瘤的胃肠道，包括30腺癌（ADCA），29鳞状细胞癌（食管和肛门），和39神经内分泌癌，和60肿瘤的胰腺，包括22 ADCA，26胰腺神经内分泌肿瘤，和12胰腺腺泡细胞癌。两个对照组的32例HCC和16例肝内胆管癌也用GPC 3染色。虽然大多数（7/12，58.5%）腺泡细胞癌GPC 3阳性，胰腺ADCA和神经内分泌肿瘤GPC 3阴性。此外，27.5%（8/29）的鳞状细胞癌、20%（6/30）的ADCA和2.5%（1/39）的胃肠道神经内分泌癌对GPC 3呈免疫反应性。HCC中75%（24/32）GPC 3阳性，而胆管癌中GPC 3阴性。虽然仅在HCC中观察到GPC 3阳性和低分化之间的显著相关性，但GPC 3表达与肿瘤大小无关。总之，14%的胃肠道和胰腺癌/肿瘤（特别是胰腺腺泡细胞癌）可以通过免疫组化表达GPC 3。由于这些肿瘤通常转移到肝脏，这为在评估累及肝脏的肿瘤时区分HCC和转移性癌提供了潜在的陷阱。(C)2013 Elsevier Inc. All rights reserved.
Glypican-3 (GPC3) is a plasma membrane bound proteoglycan that can be overexpressed in certain malignancies but has been particularly linked to hepatocellular carcinoma (HCC). GPC3 is currently used as an immunohistochemical marker for HCC, but its expression in epithelial neoplasms of the gastrointestinal (GI) tract and pancreas, a common source of liver metastasis, has not been studied in detail. In this study, we examined GPC3 immunoreactivity in 98 neoplasms of the GI tract including 30 adenocarcinomas (ADCA), 29 squamous cell carcinomas (esophageal and anal), and 39 neuroendocrine carcinomas, and 60 neoplasms of the pancreas including 22 ADCA, 26 pancreatic neuroendocrine neoplasms, and 12 pancreatic acinar cell carcinomas. Two control groups of 32 HCCs and 16 intrahepatic cholangiocarcinomas were also stained with GPC3. Although most (7/12, 58.5%) acinar cell carcinomas were GPC3 positive, pancreatic ADCA and neuroendocrine neoplasms were GPC3 negative. In addition, 27.5%, (8/29) of squamous cell carcinomas, 20% (6/30) of ADCA, and 2.5% (1/39) of neuroendocrine carcinomas of the GI tract were immunoreactive for GPC3. HCC was positive for GPC3 in 75% (24/32) of cases but cholangiocarcinoma was negative. While significant correlation between GPC3 positivity and poor differentiation was observed in HCC only, GPC3 expression did not correlate with tumor size. In conclusion, 14% of GI tract and pancreatic carcinomas/neoplasms (particularly pancreatic acinar cell carcinoma) can express GPC3 by immunohistochemistry. As these tumors commonly metastasize to the liver, this offers a potential pitfall in differentiating between HCC and metastatic carcinoma when evaluating tumors involving the liver. (C) 2013 Elsevier Inc. All rights reserved.