Alteration of high density lipoprotein subfractions of nonhuman primates fed fish-oil diets. Selective lowering of HDL subfractions of intermediate size and density.
Alteration of high density lipoprotein subfractions of nonhuman primates fed fish-oil diets. Selective lowering of HDL subfractions of intermediate size and density.
复制标题
食用鱼油饮食的非人类灵长类动物的高密度脂蛋白亚组分的变化。
DOI：
10.1161/01.atv.7.1.71
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Bullock,BC
中科院分区：
文献类型：
--
作者：
Parks,JS;Martin,JA;Sonbert,BL;Bullock,BC
给两组成年雄性非洲绿狮猴（每组 12 只动物）喂食致动脉粥样硬化饮食，其中脂肪热量占总热量的 40%，其中一半脂肪热量来自猪油或鲱鱼油，且胆固醇含量为 0.8 毫克/千卡，为期 10 个月，以确定此类饮食对脂蛋白浓度、组成和分布的影响。采用这种饮食 8 个月后，通过超速离心和琼脂糖柱色谱从动物血浆中分离出脂蛋白，并对这些脂蛋白进行化学表征。与猪油喂养组相比，油饲鲱鱼组的血浆总胆固醇（降低33％）、HDL胆固醇（降低33％）和apo A-I（降低30％）浓度以及LDL分子量（降低15％）显着降低。尽管鱼油饮食导致 LDL 胆固醇浓度平均降低（降低 34%），但这种降低并不具有统计学意义。两组动物血浆低密度脂蛋白和高密度脂蛋白的脂质和脱辅基蛋白组成相似。通过梯度凝胶电泳和密度梯度超速离心测定 HDL 亚级分的分布。与猪油喂养组相比，鱼油组中等大小（80 至 88 A）HDL 亚组分的蛋白质显着减少（减少 46%）。通过密度梯度超速离心，用鱼油喂养的猴子的 d = 1.10-1.13 g/ml 亚级分中的蛋白质浓度降低了 35% 至 40%。因此，与猪油喂养的动物相比，用鱼油喂养非人类灵长类动物会选择性降低中等大小和密度的 HDL 亚组分的浓度。（摘要截断为 250 字）
Two groups of adult male African green grivet monkeys (12 animals in each group) were fed atherogenic diets containing 40% of total calories as fat with half of the fat calories derived from lard or menhaden oil and 0.8 mg cholesterol/kcal for a period of 10 months to ascertain the effect of such diets on lipoprotein concentrations, composition, and distribution. After 8 months on this diet, the animals' lipoproteins were isolated from their plasma by ultracentrifugation and agarose column chromatography, and these were characterized chemically. Compared with the lard-fed group, the menhaden oil-fed animals had significantly lower total plasma cholesterol (decreases 33%), HDL cholesterol (decreases 33%) and apo A-I (decreases 30%) concentrations and LDL molecular weights (decreases 15%). Although the fish-oil diets resulted in an average similar reduction of LDL cholesterol concentrations (decreases 34%), the decrease was not statistically significant. The lipid and apoprotein compositions of plasma LDL and HDL were similar between the two groups of animals. The distribution of HDL subfractions was determined by gradient gel electrophoresis and density gradient ultracentrifugation. The fish-oil group had a significant reduction in protein (decreases 46%) of HDL subfractions of intermediate size (80 to 88 A) compared to the lard-fed group. By density gradient ultracentrifugation, there was a 35% to 40% lower concentration of protein in the d = 1.10-1.13 g/ml subfraction of fish-oil-fed monkeys. Thus, feeding fish oil to nonhuman primates results in a selective decrease in the concentration of HDL subfractions of intermediate size and density compared to lard-fed animals.(ABSTRACT TRUNCATED AT 250 WORDS)