PEPTIDE CONTRIBUTES TO THE SPECIFICITY OF POSITIVE SELECTION OF CD8+ T-CELLS IN THE THYMUS
PEPTIDE CONTRIBUTES TO THE SPECIFICITY OF POSITIVE SELECTION OF CD8+ T-CELLS IN THE THYMUS
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DOI：
10.1016/0092-8674(93)90281-t
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发表时间：
1993-06-04
期刊：
影响因子：
64.5
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
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作者：
ASHTONRICKARDT, PG;VANKAER, L;TONEGAWA, S
编码与抗原加工相关的肽转运蛋白（TAP 1）的基因缺陷的小鼠，其MHC I类分子的表面表达水平显著降低，CD 8 + T细胞很少。添加I类结合肽培养的胎儿胸腺从TAP 1突变小鼠总是允许救援I类表达，而只有一些这些肽促进CD 8 + T细胞，这是多克隆和特异性的肽-MHC复合物的阳性选择。当参与与TCR相互作用的残基被改变时，非选择性肽被转化为选择性肽的混合物。来自C57 BL/6胸腺的自身肽的混合物在产生相对低的I类表面表达的浓度下诱导CD 8 + T细胞的阳性选择。这些观察结果的含义是，自身肽决定，在一定程度上，由CD 8 + T细胞表现出的特异性的剧目。
Mice deficient in the gene encoding the peptide transporter associated with antigen processing (TAP1) have drastically reduced levels of surface expression of MHC class I molecules and few CD8+ T cells. Addition of class I binding peptides to cultured fetal thymi from TAP1 mutant mice invariably allowed the rescue of class I expression, while only some of these peptides promoted the positive selection of CD8+ T cells, which were polyclonal and specific for the peptide-MHC complex. A nonselecting peptide was converted to a mixture of selecting peptides when the residues involved in the interaction with TCRs were altered. A mixture of self-peptides derived from C57BL/6 thymi induced positive selection of CD8+ T cells at concentrations that gave relatively low class I surface expression. The implication of these observations is that self-peptides determine, in part, the repertoire of specificities exhibited by CD8+ T cells.