Desiccation induces varied responses within a soil bacterial genus
Desiccation induces varied responses within a soil bacterial genus
复制标题
DOI：
10.1111/1462-2920.16494
复制
发表时间：
2023-09-04
影响因子：
5.1
通讯作者：
Martiny, J. B. H.
中科院分区：
文献类型：
--
作者：
Scales, N. C.;Huynh, K. T.;Martiny, J. B. H.
干燥通过提高破坏性活性氧的水平和诱导DNA链断裂来影响微生物中的一系列生理过程。为了应对干燥引起的压力，微生物进化出了特殊的机制来帮助它们生存。在这里，我们进行了128天的实验室干燥实验，从三个分支的丰富的土壤细菌，短杆菌的九个菌株。我们在三个时间点对每个菌株的RNA进行测序，以研究它们的反应。短杆菌对干燥有很强的抵抗力，比大肠杆菌和一种著名的耐DNA损伤细菌耐辐射球菌都要持久。然而，在属内，菌株之间的存活率也存在10倍的差异。转录组学分析揭示了属内共有的反应，包括参与DNA损伤修复、渗透压调节剂产生和外排泵的基因的上调，以及三个分支特有的途径和基因的上调。例如，海藻糖合成基因otsB，伴侣groEL，和氧清除剂katA都被发现在一个或两个分支，但不是第三个。在这里，我们提供的证据，密切相关的菌株相当大的变化，进一步阐明系统发育的干燥耐受性的保护仍然是微生物生态学家的一个重要目标。
Desiccation impacts a suite of physiological processes in microbes by elevating levels of damaging reactive oxygen species and inducing DNA strand breaks. In response to desiccation-induced stress, microbes have evolved specialized mechanisms to help them survive. Here, we performed a 128-day lab desiccation experiment on nine strains from three clades of an abundant soil bacterium, Curtobacterium. We sequenced RNA from each strain at three time points to investigate their response. Curtobacterium was highly resistant to desiccation, outlasting both Escherichia coli and a famously DNA damage-resistant bacterium, Deinococcus radiodurans. However, within the genus, there were also 10-fold differences in survival rates among strains. Transcriptomic profiling revealed responses shared within the genus including up-regulation of genes involved in DNA damage repair, osmolyte production, and efflux pumps, but also up-regulation of pathways and genes unique to the three clades. For example, trehalose synthesis gene otsB, the chaperone groEL, and the oxygen scavenger katA were all found in either one or two clades but not the third. Here, we provide evidence of considerable variation in closely related strains, and further elucidation of the phylogenetic conservation of desiccation tolerance remains an important goal for microbial ecologists.