Larsenimonas suaedae sp. nov., a moderately halophilic, endophytic bacterium isolated from the euhalophyte Suaeda salsa.
Larsenimonas suaedae sp. nov., a moderately halophilic, endophytic bacterium isolated from the euhalophyte Suaeda salsa.
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灰霉病菌
DOI：
10.1099/ijsem.0.001126
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发表时间：
2016
影响因子：
2.8
通讯作者：
Meixue Dai
中科院分区：
文献类型：
--
作者：
Zhi;Hong;Chun;Qi Chen;Guo;Hai;Meixue Dai
从东营真盐藻中分离到一株中等嗜盐、革兰氏染色阴性、不形成内孢子的内生细菌ST307T。菌株ST307T为好氧菌，呈杆状，可运动，呈橙黄色。在NaCl浓度为0.6- 20% (w/v)（最适5- 6%，w/v）、温度为5-45°C（最适35°C）、pH为5-9（最适pH为7-8）的条件下生长。它积累聚β-羟基丁酸并产生外多糖。主要脂肪酸为C18: 1ω7c/C18: 1ω6c、C16: 0和C16: 1ω7c/C16: 1ω6c。主要的脂醌为泛醌Q-9。极性脂质由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、糖氨基脂和磷酸糖氨基脂组成。DNA G+C含量为60.5 mol%。16S rRNA基因序列和连接的atpA、rpoD和secA基因序列的系统发育分析表明，该菌株属于Larsenimonas属。最近的亲缘型菌株是盐生Larsenimonas salina M1-18T。菌株ST307T与近缘种L. salina M1-18T、beijerinckichrohalobacter DSM 7218T、C. canadensis DSM 6769T、C. israelensis DSM 6768T、C. marismortui CGMCC 1.2321T、C. nigrandesensis DSM 14323T、C. salexigens DSM 3043T和C. sarecensis DSM 15547T的平均DNA-DNA亲缘值为15±2 ~ 45±1%。根据表型、化学分类和分子特征，菌株ST307T明显是Larsenimonas属的一个新种。提出以ST307T （=CGMCC 1.8902T=DSM 22428T）为类型菌株命名为Larsenimonassuaedae sp. nov.。
A moderately halophilic, Gram-stain-negative, non-endospore-forming endophytic bacterium designated strain ST307T was isolated from the euhalophyte Suaeda salsa in Dongying, China. Strain ST307T was aerobic, rod-shaped, motile and orange-yellow-pigmented. The organism grew at NaCl concentrations of 0.6-20 % (w/v) (optimum 5-6 %, w/v), at temperatures of 5-45 °C (optimum 35 °C) and at pH 5-9 (optimum pH 7-8). It accumulated poly-β-hydroxybutyric acid and produced exopolysaccharides. The major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The predominant lipoquinone was ubiquinone Q-9. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a glycoaminolipid and a phosphoglycoaminolipid. The DNA G+C content was 60.5 mol%. Phylogenetic analyses of 16S rRNA gene sequences and concatenated atpA, rpoD and secA gene sequences revealed that the strain represents a member of the genus Larsenimonas. The closest related type strain was Larsenimonas salina M1-18T. Mean DNA-DNA relatedness values between strain ST307T and the related species L. salina M1-18T, Chromohalobacter beijerinckii DSM 7218T, C. canadensis DSM 6769T, C. israelensis DSM 6768T, C. marismortui CGMCC 1.2321T, C. nigrandesensis DSM 14323T, C. salexigens DSM 3043T and C. sarecensis DSM 15547T were 15±2-45±1 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain ST307T clearly represents a novel species of the genus Larsenimonas. The name Larsenimonassuaedae sp. nov. is proposed, with ST307T (=CGMCC 1.8902T=DSM 22428T) as the type strain.
DOI：
10.1099/ijs.0.038075-0
发表时间：
2012-03-01
影响因子：
2.8
作者：
Kim, Ok-Sun;Cho, Yong-Joon;Chun, Jongsik
通讯作者：
Chun, Jongsik